Q1LUA8 (EI3EB_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 3 subunit E-B
Short name=eIF3e-B

Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6-B

Gene names

Name:	eif3eb
Synonyms:	eif3s6b
ORF Names:	si:ch211-129b17.1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome By similarity. HAMAP-Rule MF_03004
Subunit structure	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: eif3a, eif3b, eif3c, eif3d, eif3e, eif3f, eif3g, eif3h, eif3i, eif3j, eif3k, eif3l and eif3m By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity HAMAP-Rule MF_03004.
Sequence similarities	Belongs to the eIF-3 subunit E family. Contains 1 PCI domain.
Sequence caution	The sequence CAK04060.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAK04061.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Initiation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	eukaryotic translation initiation factor 3 complex Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q1LUA8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q1LUA8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     390-446: GHVVMGNNAV...NWAAQDTGFY → QENWSVLQLPRYSRRNADL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Eukaryotic translation initiation factor 3 subunit E-B HAMAP-Rule MF_03004		PRO_0000365955
Regions
	Domain	291 – 396	106	PCI
Natural variations
	Alternative sequence	390 – 446	57	GHVVM…DTGFY → QENWSVLQLPRYSRRNADL in isoform 2.		VSP_036544

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: 8E8CCD6B9C3317FD	FASTA	446	52,602
	10 20 30 40 50 60 MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSETNM VDFAMDVYKN 70 80 90 100 110 120 LYPDKEIPHS LRDKRTTVVA QLKQLQSETE PIVKMFEDPE TQRQMQSTRD GRMLFEYLAD 130 140 150 160 170 180 KHSFRQEYLD TLYRYAKFQY ECGNYSGAAE YLYFFRVLVP STDRNALSSL WGKLASEILM 190 200 210 220 230 240 QNWEAAMEDL TRLRETIDNN TVSSPLQSLQ QRTWLIHWSL FVFFNHPKGR DNIIELFLYQ 250 260 270 280 290 300 PQYLNAIQTM CPHILRYLST AVITNKDVRK RRQVLKDLVK VIQQESYTYK DPITEFVECL 310 320 330 340 350 360 YVNFDFDSAQ RKLRECESVL VNDFFLVACL EDFIENARLF IFETFCRIHQ CISISMLADK 370 380 390 400 410 420 LNMTPEEAER WIVNLIRNAR LDAKIDSKLG HVVMGNNAVS PYQQVIEKTK SLSFRSQMLA 430 440 MNIEKKISHS NRNETPNWAA QDTGFY
	Isoform 2 [UniParc]. Checksum: 3DEC737DC942C62A	FASTA	408	48,493
	10 20 30 40 50 60 MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSETNM VDFAMDVYKN 70 80 90 100 110 120 LYPDKEIPHS LRDKRTTVVA QLKQLQSETE PIVKMFEDPE TQRQMQSTRD GRMLFEYLAD 130 140 150 160 170 180 KHSFRQEYLD TLYRYAKFQY ECGNYSGAAE YLYFFRVLVP STDRNALSSL WGKLASEILM 190 200 210 220 230 240 QNWEAAMEDL TRLRETIDNN TVSSPLQSLQ QRTWLIHWSL FVFFNHPKGR DNIIELFLYQ 250 260 270 280 290 300 PQYLNAIQTM CPHILRYLST AVITNKDVRK RRQVLKDLVK VIQQESYTYK DPITEFVECL 310 320 330 340 350 360 YVNFDFDSAQ RKLRECESVL VNDFFLVACL EDFIENARLF IFETFCRIHQ CISISMLADK 370 380 390 400 LNMTPEEAER WIVNLIRNAR LDAKIDSKLQ ENWSVLQLPR YSRRNADL

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX957242 Genomic DNA. Translation: CAK04060.1. Sequence problems. BX957242 Genomic DNA. Translation: CAK04061.1. Sequence problems.
IPI	IPI00610058. IPI00770623.
UniGene	Dr.5119.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q1LUA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dre:561049.
Organism-specific databases
CTD	561049.
ZFIN	ZDB-GENE-050208-283. eif3eb.
Phylogenomic databases
GeneTree	ENSGT00390000002661.
HOGENOM	HOG000036697.
HOVERGEN	HBG001097.
KO	K03250.
Gene expression databases
ArrayExpress	Q1LUA8.
Bgee	Q1LUA8.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_03004. eIF3e.
InterPro	IPR016650. eIF3e. IPR019010. eIF3e_N. IPR000717. PCI_dom. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR10317. PTHR10317. 1 hit.
Pfam	PF09440. eIF3_N. 1 hit. PF01399. PCI. 1 hit. [Graphical view]
PIRSF	PIRSF016255. eIF3e_su6. 1 hit.
SMART	SM00088. PINT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20883737.

Entry information

Entry name

EI3EB_DANRE

Accession

Primary (citable) accession number: Q1LUA8
Secondary accession number(s): Q1LUA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	May 29, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program