Q1LU68 (METE_BAUCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BCI_0017

Organism

Baumannia cicadellinicola subsp. Homalodisca coagulata [Complete proteome] [HAMAP]

Taxonomic identifier

374463 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Candidatus Baumannia

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017224
Sites
	Metal binding	646	1	Zinc By similarity
	Metal binding	648	1	Zinc By similarity
	Metal binding	731	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LU68 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 2EDBEE7151AC2A4C	FASTA	764	87,406
	10 20 30 40 50 60 MTILSHILGF PRIGLHRELK RAQESYWDGH ITQQQLLNTG RELRACHWQQ QKQAGLNMLP 70 80 90 100 110 120 VGDFAWYDHV LTTSLMLDNV PVRHRNDDGS SNIDTLYRIG RGNAPTGQPV VASEMTKWFN 130 140 150 160 170 180 TNYHYIVPEF TVGQQFRLGW TQLIEEVDEA LALGYNIKPI LLGPISYLWL GKAKEKHFDR 190 200 210 220 230 240 LSLLSALLPV YQQILALLAQ RGIEWVQIDE PALVLELPTA WCNAYYDAYT DALQGYSKLL 250 260 270 280 290 300 LTTYFDSIGH HLNIITNLSV HGLHVDLIAG NDDVEVLHRT LPSNWVLSAG VINGRNVWRA 310 320 330 340 350 360 DLPSKFKQLR SLVGQRELWV GSSCSLLHSP IDLSIETRMD PEVKSWFAFT IQKCNELKLL 370 380 390 400 410 420 CNALNRLDDT KYQAILADYS APLWARQTSE KVHNRQVKIR TEQITENHSQ RQQHYLDRIN 430 440 450 460 470 480 VQRARFNLPI LPTTTIGSFP QTTAIRSLRL DLKSGRIDNE YYKNAICNHI KQAITEQDAL 490 500 510 520 530 540 GLDVLVHGEA ERNDMVEYFG EHLNGFIFTQ NGWVQSYGSR CVKPPIIIGD ISRPKPITVE 550 560 570 580 590 600 WAWYAQSLTQ KPVKGMLTGP VTILCWSFPR EDLDRKTIAR QIALALRDEV IDLENAGIGI 610 620 630 640 650 660 IQIDEPALRE GLPLKYSAWK EYLTWAVEAF RLNAAVAKNN TQIHTHMCYS EFNDIMDSII 670 680 690 700 710 720 AMDADVITIE TSRSNMKLLD TFKQYQYPND IGPGVYDIHS PNIPSEDDII QLLRKAAQVI 730 740 750 760 PIKRLWVNPD CGLKTRTWLE TRKALSNMVN AAHKLRREEE YNRS

References

[1]

"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters." Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A. PLoS Biol. 4:1079-1092(2006) [PubMed: 16729848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000238 Genomic DNA. Translation: ABF14113.1.
RefSeq	YP_588496.1. NC_007984.1.
3D structure databases
ProteinModelPortal	Q1LU68.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LU68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4056528.
GenomeReviews	Gene locus BCI_0017 in contig CP000238_GR.
KEGG	bci:BCI_0017.
PATRIC	21073521. VBIBauCic75062_0017.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q1LU68.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BCIC186490:BCI_0017-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BAUCH

Accession

Primary (citable) accession number: Q1LU68

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program