Reviewed, UniProtKB/Swiss-Prot Q1LTQ4 (SYA_BAUCH)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BCI_0205

Organism

Baumannia cicadellinicola subsp. Homalodisca coagulata [Complete proteome] [HAMAP]

Taxonomic identifier

374463 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Candidatus Baumannia

Protein attributes

Sequence length	884 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 884	884	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347505

Sequences

	Sequence		Length	Mass (Da)
	Q1LTQ4-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 180A39380BE73293	FASTA	884	99,561
	10 20 30 40 50 60 MSNNSTAELR QEFLNFFHQK GHKIVASSSL VSHNDPTLLF TNAGMNQFKD VFLGLDKRLY 70 80 90 100 110 120 QSATTAQRCI RAGGKHNDLE KVGYTARHHT FFEMLGNFSF GAYFKCEAIQ LAWELLTGGQ 130 140 150 160 170 180 WFNLPKDKLW VTVYATDDET YNIWAKQINI PRERIVRIGD NKGKAYASDN FWQMGDIGPC 190 200 210 220 230 240 GPCSEIFFDH GNHLFGAPPG SQGEYGDRYI EIWNLVFIQF NRQMDGTLIP LQKPSVDTGM 250 260 270 280 290 300 GLERIAAVLQ HVNSNYDIDI FSQLIKAVAD VTKATNTSSL QVIADHIRSC AFLVSDGVVP 310 320 330 340 350 360 SQDGRGYVLR HIIRRAIRHG YMLGARDAFF YKLVAPLIEV MGSTADQLKQ QQNMVEQVLR 370 380 390 400 410 420 TEEEQFSQTL ERGLFMLEQV LTKLTGDTLD GETAFSLYDT YGLPIYLTTD ICRERNIKVD 430 440 450 460 470 480 ENGFKSAMEV QRNRAREASR FSYKYNSMLS INQYTRFLGY KHLKSQGKVT ALYLNDQSVE 490 500 510 520 530 540 VIYHSEKAIV ILDYTPFYGE SGGQIGDSGK ITAANGIFEV IDTKKYGNAL GHLGMLNYGE 550 560 570 580 590 600 LRIGDQVTAK VNQLRRDRIS VNHSATHLLQ SALRQLLGKH VVQRGSLVHD QYLRFDFSHH 610 620 630 640 650 660 QAMQVEQIHE VEKIINQQIR RNLIIKTDMM TLEAAQNQGA ISVLDHQYES SQVRVLSIGD 670 680 690 700 710 720 FSTELCCGTH ASRTGDIGLF WISSESGIAL GIRRIEAITG EFALKNLHQQ SNLVQQISQL 730 740 750 760 770 780 FKNDGSNLVE KVRGLQNHIK QLESKYQQLK SKQVAQEIAV LSRKISKMHG TQVLVSKLEN 790 800 810 820 830 840 IEPKIMRHIV DEIKHQLGSV VIILANINLV DGKVNLIAGV TDDLTNRIQA SDIIRTLIQK 850 860 870 880 FGGRGGGSKK IAYAGSITTN ELSTVLYYIE KIVAVKFTES QEMN

References

[1]

"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters." Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A. PLoS Biol. 4:1079-1092(2006) [PubMed: 16729848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000238 Genomic DNA. Translation: ABF13997.1.
RefSeq	YP_588660.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LTQ4.
Genome annotation databases
GeneID	4056745.
GenomeReviews	Gene locus BCI_0205 in contig CP000238_GR.
KEGG	bci:BCI_0205.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1LTQ4.
OMA	ASGKHND.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BAUCH

Accession

Primary (citable) accession number: Q1LTQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 30, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)