Reviewed, UniProtKB/Swiss-Prot Q1LTP2 (CYSI_BAUCH)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	BCI_0217

Organism

Baumannia cicadellinicola subsp. Homalodisca coagulata [Complete proteome] [HAMAP]

Taxonomic identifier

374463 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Candidatus Baumannia

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_0000292958
Sites
	Metal binding	430	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	436	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	475	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	479	1	Iron (siroheme axial ligand) By similarity
	Metal binding	479	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LTP2-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 241F9CAD6D6E7ED4	FASTA	566	63,931
	10 20 30 40 50 60 MNKKIPKAIL SDNERLKVAS NFLRGTIAQD LQDNITGGFK GDNIQLIRFH GMYQQDDRDL 70 80 90 100 110 120 RVERTCQKLE PLINMMLRCR LPGGIITPQQ WLGIDSFATK HTLYGSIRLT TRQTFQFHGV 130 140 150 160 170 180 LKPNLKNMHK LLHSLGLDSI ATAGDMNRNV LCTSNPVESV LHQQVCNYAK MISEHFLPKT 190 200 210 220 230 240 RAYAEIWLDG EKTETTEQEP ILGANYLPRK FKISIVVPPL NDVDLHANDL NFIAISNLGQ 250 260 270 280 290 300 LVGFNVLVGG GLAMTHNDKS TYPRTASELG YISVVDTIKI AEAVITTQRD LGDRSNRKHA 310 320 330 340 350 360 KTKYTIERVG VEFFKKEVEV RAGIKFKHIR PYSFTERGDR FGWVQGIDNQ WHLTLFIENG 370 380 390 400 410 420 RIIDDSHRKL KTGMLEIARI HQGDFRITAN QNIIIAGVEK KHKATIELLA RQYGLINNNI 430 440 450 460 470 480 TLQRKASMAC VAFPTCPLAM AEAERFLPQF VTKVENIMSR HGLGQEKIIL RVTGCPNGCG 490 500 510 520 530 540 RAMLAEIGLV GKTIGRYNLY LGGDSIGTRI PRIYRENLTE EEILSIINDT TGRWARERQP 550 560 DESYGDYVVR AGIIRPVINS ALDFHN

References

[1]

"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters." Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A. PLoS Biol. 4:1079-1092(2006) [PubMed: 16729848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000238 Genomic DNA. Translation: ABF13903.1.
RefSeq	YP_588672.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4056488.
GenomeReviews	Gene locus BCI_0217 in contig CP000238_GR.
KEGG	bci:BCI_0217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1LTP2.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_BAUCH

Accession

Primary (citable) accession number: Q1LTP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	May 30, 2006
Last modified:	November 25, 2008
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)