Reviewed, UniProtKB/Swiss-Prot Q1LTN7 (PYRG_BAUCH)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	BCI_0222

Organism

Baumannia cicadellinicola subsp. Homalodisca coagulata [Complete proteome] [HAMAP]

Taxonomic identifier

374463 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Candidatus Baumannia

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	CTP synthase HAMAP MF_01227		PRO_0000266067
Regions
	Domain	291 – 538	248	Glutamine amidotransferase type-1
	Region	1 – 253	253	Aminator domain HAMAP MF_01227
Sites
	Active site	379	1	Nucleophile By similarity
	Active site	511	1	By similarity
	Active site	513	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LTN7-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: D2665CA19382B0B3	FASTA	543	60,718
	10 20 30 40 50 60 MKTNYIFVTG GVVSSLGKGI AVASLAAILE ARGLKVTIMK LDPYINVDPG TISPIQHGEV 70 80 90 100 110 120 FVTEDGAETD LDLGHYERFI RTKMSRRNNF TTGRIYFDVL HKERRGDYLG ATIQVIPHIT 130 140 150 160 170 180 NTIKQRIIEC GEGHDVVLVE IGGTVGDIES LPFLEAIRQM AGEVGRDHTL YMHLTLVPYI 190 200 210 220 230 240 KAAGEVKTKP TQHSVKELLS IGIQPDVLIC RSDRAVPSNE RAKIALFCNV SEKAVISLKD 250 260 270 280 290 300 VDSIYKIPAL LKSQYLDDYI CERFSLKCPK ANLSEWEQVI YQQANPVGEV TVGIVGKYID 310 320 330 340 350 360 LPDAYKSVIE ALHHAGLKNR IAVNICLIHS QDVETRSVKI LQDLDAILIP GGFGYRGVEG 370 380 390 400 410 420 KIMTAQYARE KQIPYLGICL GMQVAIVEFA RHVAGMPEAN STEFVSDCKY PVVALITEWY 430 440 450 460 470 480 EENNNRKLSN LGGTMRLGSQ PCKLTYGSLA YQIYGKTIIM ERHRHRYEVN NMLLKQIEAA 490 500 510 520 530 540 GLRVAGLSED YKLVEMIEYP AHPWFIASQF HPEFNSTPRD GHPLFTGFIK AASEYQKKQL NKM

References

[1]

"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters." Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A. PLoS Biol. 4:1079-1092(2006) [PubMed: 16729848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000238 Genomic DNA. Translation: ABF13830.1.
RefSeq	YP_588677.1.
3D structure databases
SMR	Q1LTN7. Positions 1-539.
ModBase	Search...
Genome annotation databases
GeneID	4056160.
GenomeReviews	Gene locus BCI_0222 in contig CP000238_GR.
KEGG	bci:BCI_0222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1LTN7.
OMA	Q1LTN7. EFNNAYR.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_BAUCH

Accession

Primary (citable) accession number: Q1LTN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)