Q1LTM9 (SYL_BAUCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	BCI_0232

Organism

Baumannia cicadellinicola subsp. Homalodisca coagulata [Complete proteome] [HAMAP]

Taxonomic identifier

374463 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Candidatus Baumannia

Protein attributes

Sequence length	861 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049_B
Subcellular location	Cytoplasm HAMAP MF_00049_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW leucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 861	861	Leucine--tRNA ligase HAMAP MF_00049_B		PRO_1000009296
Regions
	Motif	42 – 52	11	"HIGH" region HAMAP MF_00049_B
	Motif	620 – 624	5	"KMSKS" region HAMAP MF_00049_B
Sites
	Binding site	623	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LTM9 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 151E545728E934B5	FASTA	861	99,335
	10 20 30 40 50 60 MQEFYDPKNI ESTIQQYWHE NNTFTVTEDF SKEKYYCLSM LPYPSGNLHM GHVRNYTIGD 70 80 90 100 110 120 VLSRYHRMLG KNVMQPIGWD AFGLPAERAA LKNQTAPATW TYANIETMKK QLKQLGFSYD 130 140 150 160 170 180 WSREITTCRP EYYRWEQWFF IQLYEKGLVY KKTSFVNWCS NDQTVLANEQ VIDGCCWRCG 190 200 210 220 230 240 APIMLKDIPQ WFLKITAYAD QLLHDLDKLD GWPEQIKNMQ RNWIGRSEGI NITFQVIDMK 250 260 270 280 290 300 ETLTIYTTRP DTLMGVTYLS IAINHHLAQQ AANNNRLLSD FIEHSRPTKL SEAEIVKVNR 310 320 330 340 350 360 VKTGIFTGLY AIHPLTEEKL PIWVTNFVLM DYGTGAIMAV PGHDQRDWDF ARQYNLPVKN 370 380 390 400 410 420 IIRNIDGSKP TISGIIPEGI LYNSGEFNGL RSLEASKIIT DILVARGIGE TKVNYRLRDW 430 440 450 460 470 480 VISRQRYWGT PIPMMTLEDG TVVPTPVDQL PVILPEYLLI NSISNPLKDD HLWMKTNYNN 490 500 510 520 530 540 NIATRETDTF DTFMESSWYY ARYTCPNYDQ GMLDTTAANY WLPIDQYIGG IEHAIMHLMY 550 560 570 580 590 600 FRFYHKLLRD AGMLTSDEPT IRILCQGMVL ADSFYYISCT TGERIWVSPI NVRVQRDEKG 610 620 630 640 650 660 NIINAIDLQG HHLVYAGTIK MSKSKNNSID PLTMVEKYGA DTIRLFIMFA SPVTMALEWR 670 680 690 700 710 720 ESGVEGANRF LKRLWKLTYD HIQRGKVIKL DLAAMSNDNK ILRRELHQTI AKVTDDISRR 730 740 750 760 770 780 YAFNTAIAAL MEITNKLMHA SYHSQQDRAI VQEALLAVVR MLYPFTPHLC FKLWQALNGE 790 800 810 820 830 840 GDIDNAPWPI VDQLALVEDT NLIVIQINGR FRSKIIVPVS ADKALIIERA SKEKLVAKYL 850 860 EGTKVQKIIY VPGKLLNLVL K

References

[1]

"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters." Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A. PLoS Biol. 4:1079-1092(2006) [PubMed: 16729848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000238 Genomic DNA. Translation: ABF13994.1.
RefSeq	YP_588685.1. NC_007984.1.
3D structure databases
ProteinModelPortal	Q1LTM9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LTM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4056741.
GenomeReviews	Gene locus BCI_0232 in contig CP000238_GR.
KEGG	bci:BCI_0232.
PATRIC	21073965. VBIBauCic75062_0221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HBG693845.
OMA	FWHKVLF.
PhylomeDB	Q1LTM9.
Enzyme and pathway databases
BioCyc	BCIC186490:BCI_0232-MONOMER.
Family and domain databases
HAMAP	MF_00049_B. Leu_tRNA_synth_B. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 3 hits.
KO	K01869.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 5 hits. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. LeuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_BAUCH

Accession

Primary (citable) accession number: Q1LTM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program