Reviewed, UniProtKB/Swiss-Prot Q1LT75 (SYM_BAUCH)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BCI_0396

Organism

Baumannia cicadellinicola subsp. Homalodisca coagulata [Complete proteome] [HAMAP]

Taxonomic identifier

374463 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Candidatus Baumannia

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_1000075579
Regions
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00098
	Motif	332 – 336	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	146	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	162	1	Zinc By similarity
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LT75-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 8BE3640077799B5B	FASTA	547	62,987
	10 20 30 40 50 60 MIQVASKILV TCALPYANGS LHLGHMLEHI QADIWVRYQR IRGKQVYFIC ADDAHGTPIM 70 80 90 100 110 120 LKAKQSGITP EAMINKINQE HQTDLAQFEI SYDNYYSTHS DENRELVISI YNTLKENGLI 130 140 150 160 170 180 KKRMISQLYD PIHNIFLPDR FVKGYCPRCK LPDQYGDNCE ICGATYHPTD LIDPKSTLSG 190 200 210 220 230 240 VTPVISKSKH LFFDLPVFSE VLRAWTRSGA LQEQVANKMQ EWFDMGLQQW DISRDAPYFG 250 260 270 280 290 300 FEVPDTPGKY FYVWLDAPIG YIGAFKNLCN KRNDIIFDEF WHLSSKADLY HFIGKDITYF 310 320 330 340 350 360 HGIFWPAILE GSKLRKPTNL FVHGYVTING AKMSKSRGTL IKASTYLAHL DASYLRYYYA 370 380 390 400 410 420 TKLSSDINDI DLNFNDFVNR VNADIINKVI NLAARNASFI QKYFDNKLSA TIEDQYLYDY 430 440 450 460 470 480 FVTASVSIGE AFNNRETSRA IREIMILADR ANVYIHKKEP WVVAKNKYYQ QDLHNICSMG 490 500 510 520 530 540 INLFRLLMTY LQPVLPNLAI QAEAFLNTKL NWDSMVIPLT NHKISPFKTL LQRITLSQVK AMIDATH

References

[1]

"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters." Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A. PLoS Biol. 4:1079-1092(2006) [PubMed: 16729848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000238 Genomic DNA. Translation: ABF14300.1.
RefSeq	YP_588839.1.
3D structure databases
SMR	Q1LT75. Positions 5-546.
ModBase	Search...
Genome annotation databases
GeneID	4056275.
GenomeReviews	Gene locus BCI_0396 in contig CP000238_GR.
KEGG	bci:BCI_0396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1LT75.
OMA	Q1LT75. DFSWREF.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BAUCH

Accession

Primary (citable) accession number: Q1LT75

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)