Reviewed, UniProtKB/Swiss-Prot Q1LT62 (PYRD_BAUCH)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BCI_0409

Organism

Baumannia cicadellinicola subsp. Homalodisca coagulata [Complete proteome] [HAMAP]

Taxonomic identifier

374463 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Candidatus Baumannia

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024153
Sites
	Active site	175	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LT62-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: B00B0E763B82D580	FASTA	336	36,873
	10 20 30 40 50 60 MLYPLIRQFL FQLDPEKAHE LTIQQLKRIA GTPLEYLVRQ SVPTKAVTCM GIAFKNPLGL 70 80 90 100 110 120 AAGLDKNGEC IDALGAMGFG FIEVGTVTPH AQIGNEKPRL FRLTKAYGLI NRMGFNNKGV 130 140 150 160 170 180 DNLVNNIQKS HFGGVLGINI GKNKDTPIEQ GKDDYLICME KVYPYANYIA VNISSPNTPQ 190 200 210 220 230 240 LTTLQSGEIL DDLLRAIKDK QAKLQEYHHK YVPIAVKITP DMTESELIQM ADLLVQHKID 250 260 270 280 290 300 GIIATNTTTS RELVHGLDHS SQSGGLSGRP LQLMSTEVIR ILSSKLQGKL PIIGVGGIDS 310 320 330 LISAREKMAA GATLIQIYSS FIFHGPRLIK DIISDL

References

[1]

"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters." Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A. PLoS Biol. 4:1079-1092(2006) [PubMed: 16729848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000238 Genomic DNA. Translation: ABF14134.1.
RefSeq	YP_588852.1.
3D structure databases
SMR	Q1LT62. Positions 1-336.
ModBase	Search...
Genome annotation databases
GeneID	4056320.
GenomeReviews	Gene locus BCI_0409 in contig CP000238_GR.
KEGG	bci:BCI_0409.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1LT62.
OMA	Q1LT62. NAEQQGG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BAUCH

Accession

Primary (citable) accession number: Q1LT62

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)