Q1LSV9 (QUEF_BAUCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	BCI_0525

Organism

Baumannia cicadellinicola subsp. Homalodisca coagulata [Complete proteome] [HAMAP]

Taxonomic identifier

374463 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Candidatus Baumannia

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.
Sequence caution	The sequence ABF13980.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000247702

Sequences

	Sequence		Length	Mass (Da)
	Q1LSV9 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: F23E4B62CCF4A683	FASTA	283	33,067
	10 20 30 40 50 60 MTDQQYQLLQ KFKLDKSKIS YRTHYNPMLL QPIPRTIHRE LLGISSISLP FQGADFWTLY 70 80 90 100 110 120 ELSWLNKYGL PQVAIGEMII DATSDNLIES QSLKLYLNSI NQTCFSSEQE LRNTLITDIT 130 140 150 160 170 180 KCINSQVKIT IHSLSQWMTV PILEFTGECI DNQSIQIDND NYIFNSNLLV NSVEREKVKE 190 200 210 220 230 240 TLVSHLLKSN CPITNQPDWG SVQISYYGMR INREALLRYL ISFRKYKIFH EQCVEQIYCD 250 260 270 280 IMQFCLPNTL SVYVRYNRRG GLDINPWRSN ISYSPVNRPL ARQ

References

[1]

"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters." Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A. PLoS Biol. 4:1079-1092(2006) [PubMed: 16729848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000238 Genomic DNA. Translation: ABF13980.1. Different initiation.
RefSeq	YP_588955.1. NC_007984.1.
3D structure databases
ProteinModelPortal	Q1LSV9.
SMR	Q1LSV9. Positions 21-283.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LSV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4056410.
GenomeReviews	Gene locus BCI_0525 in contig CP000238_GR.
KEGG	bci:BCI_0525.
PATRIC	21074559. VBIBauCic75062_0497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG289139.
PhylomeDB	Q1LSV9.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	BCIC186490:BCI_0525-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BAUCH

Accession

Primary (citable) accession number: Q1LSV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program