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Q1LSS9

- SYI_BAUCH

UniProt

Q1LSS9 - SYI_BAUCH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Baumannia cicadellinicola subsp. Homalodisca coagulata
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (30 May 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBCIC374463:GI6Q-556-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BCI_0556
    OrganismiBaumannia cicadellinicola subsp. Homalodisca coagulata
    Taxonomic identifieri374463 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaCandidatus Baumannia
    ProteomesiUP000002427: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_1000022042Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi374463.BCI_0556.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1LSS9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1LSS9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDYKNTLNL PKTQFAMRGN LAIREPIMLK RWHQQDLYQL ICQATQGKKT    50
    FFLHDGPPYA NGSIHIGHSV NKILKDIIIK SKRLMGYCSP YIPGWDCHGL 100
    PIELKVEQLI GKPGKKVSAS EFIIACRNYA SEQVTWQKKD FIRLGVLGDW 150
    DNIYRTMDFH TEANIIRTLS KIIENGHVYQ GNKPVHWCID CRSALAEAEV 200
    EYYDYTSPSI YVIFAATNTH DVAARFGIPN VLSSISFLVW TTTPWTIPAN 250
    RAISIHPNLN YQLVKVNQQG FILAADLVTS VLTYLGIQNW TVVKNIKGYV 300
    LELLRFSHPF MKFDVPVVLS NHVTINVGTG VVHTSPSHGP DDYLIGREYN 350
    LEIVNIVGPD GCYLPGTFSL LDGTSVYQSN QTVISLLKDR GALLHTGTIQ 400
    HSYPHCWRHK TPLIFRATPQ WFISMDKKKL RQQSLKEIKK IQWIPSNSQA 450
    SITNMVNNRQ DWCISRQRIW GVPMSLFVHN HTKKLHPQTS EIMEYVAKQV 500
    EKKGIQAWWD LDPIKILGDD IVNYSKINDI LDVWFDSGST HSSVINAQTE 550
    FANHEIDMYL EGADQHRGWF MSSLISSTAI KGKAPYKTVI THGFAVDSNG 600
    RKMSKSIGNV VSPQQVVDKL GADILRLWVA STNYTDDMTI SDEILKRSVD 650
    TYRRIRNTAR FLLANLNGFE PKQHSVSIDK MIILDQWAID RAQVAQDEII 700
    AAYNSYEFHS VVQRIMQFCS VEMGSFYLDI IKDRQYTTQY NSIARRSCQT 750
    ALFHIIEAMV RWIAPIISFT ADEIWGFIPG KRSPSVFIEE WYKNLSRLDA 800
    EQHMNDTYWN TLLQVRSDVN YLIEQARIKK NIGSSLETQV TLYSEPILAM 850
    QLRQLGNELH FVLLTSAVQI ADYQEADNNA LQSTRIKGLK ITLNHATGRK 900
    CQRCWHYEQD IGNNTQYPEI CGRCVINIAG NGEERKFV 938
    Length:938
    Mass (Da):107,240
    Last modified:May 30, 2006 - v1
    Checksum:i697B57DCEEB6DAAA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000238 Genomic DNA. Translation: ABF14142.1.
    RefSeqiYP_588985.1. NC_007984.1.

    Genome annotation databases

    EnsemblBacteriaiABF14142; ABF14142; BCI_0556.
    GeneIDi4056571.
    KEGGibci:BCI_0556.
    PATRICi21074621. VBIBauCic75062_0527.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000238 Genomic DNA. Translation: ABF14142.1 .
    RefSeqi YP_588985.1. NC_007984.1.

    3D structure databases

    ProteinModelPortali Q1LSS9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 374463.BCI_0556.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABF14142 ; ABF14142 ; BCI_0556 .
    GeneIDi 4056571.
    KEGGi bci:BCI_0556.
    PATRICi 21074621. VBIBauCic75062_0527.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BCIC374463:GI6Q-556-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters."
      Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A.
      PLoS Biol. 4:1079-1092(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiSYI_BAUCH
    AccessioniPrimary (citable) accession number: Q1LSS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3