Q1LS86 (ACEK_RALME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Rmet_0104

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 607	607	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000259156
Regions
	Nucleotide binding	328 – 334	7	ATP By similarity
Sites
	Active site	384	1	By similarity
	Binding site	349	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LS86 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: D190928A003C2C49	FASTA	607	71,110
	10 20 30 40 50 60 MSHFPKLLSS QIAYDVARTM LDGFDKHYRL FREVSIEAKA RFEVGDWHGL QQLQRDRIAF 70 80 90 100 110 120 YNQRVHETIV TLQDEYDAED IEDEIWQQIK LHYIGLLTNH HQPELAETFF NSVFTRIQHR 130 140 150 160 170 180 SYFNNDFIFV RPAISTEYIE NEESPIKPTY RAYYPGSREG MAACFERIVN NFQLERPFED 190 200 210 220 230 240 LKRDAGYVAR AITEHFGEFR IAPNFQVHTL SSLFFRNKTA FVIGRVINGD HTYPLVVPII 250 260 270 280 290 300 HAASGKLVLD TVLLHREQIL ILFSFAHAYF MVDMEIPSAY VTFLRDLLPR KSRAEIYTSL 310 320 330 340 350 360 GLQKQGKNLF YRDFLHHLQH SSDKFISAPG IKGLVMLVFT LPSFPYVFKV IRDFFPAPKE 370 380 390 400 410 420 TTRELVKSKY QLVKQHDRVG RMADTLEYSD VAFPLSRFDD ALVRELEQHA PSMIEYQRSK 430 440 450 460 470 480 QGEDEIVVRH VYIERRMTPL NIWLQEGTDE QVDHGILEYG NAVKELIAAN IFPGDMLYKN 490 500 510 520 530 540 FGVTRHGRVV FYDYDEIEYL TDCNVRRVPA PRNEEEEMSG EVWYTVRPHD IFPETYGTFL 550 560 570 580 590 600 LGDPRVRAAF MRHHPDFFDA AMWQHHKDRL LAGHVHDFFA YHSQERFIHR YGSGATGPAT EEPRRAA

References

[1]

"Complete sequence of the chromosome of Ralstonia metallidurans CH34." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000352 Genomic DNA. Translation: ABF06990.1.
RefSeq	YP_582259.1. NC_007973.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LS86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4036888.
GenomeReviews	Gene locus Rmet_0104 in contig CP000352_GR.
KEGG	rme:Rmet_0104.
PATRIC	20284726. VBIRalMet4734_0480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	Q1LS86.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	RMET266264:RMET_0104-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_RALME

Accession

Primary (citable) accession number: Q1LS86

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program