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Q1LS73

- BIOB_RALME

UniProt

Q1LS73 - BIOB_RALME

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Protein
Biotin synthase
Gene
bioB, Rmet_0117
Organism
Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi125 – 1251Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi156 – 1561Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi216 – 2161Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi288 – 2881Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCMET266264:GJ5G-119-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Rmet_0117
OrganismiRalstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Taxonomic identifieri266264 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002429: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthaseUniRule annotation
PRO_0000381574Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi266264.Rmet_0117.

Structurei

3D structure databases

ProteinModelPortaliQ1LS73.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1LS73-1 [UniParc]FASTAAdd to Basket

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MTQQANQTVA TISAEALRQS ARNIAAAAPK EGDAWRVDDV AALFALPFND    50
LLFRAQQVHR EHFDANTVQL STLLSIKTGG CEEDCGYCPQ SAHHDAGVKA 100
EKLMDLEAVL DAAKAAKANG ATRFCMGAAW REPKERHLEP VIDMVREVKA 150
MGLETCVTLG MLKAEQAQRL KDAGLDYYNH NLDTSPEFYG KIITTRTYQD 200
RLDTIGHVRE AGINVCCGGI VGMGEAREAR AGLIAQLANM DPYPESVPIN 250
NLVQVEGTPL AGTEALDPFE FVRTIAVARI TMPRAMVRLS AGREAMDEAL 300
QALCFMAGAN SIFYGEKLLT TGNPQADRDR ALLARLDIRA EGYAG 345
Length:345
Mass (Da):37,484
Last modified:July 28, 2009 - v2
Checksum:iBE1CEA1AD65D19E9
GO

Sequence cautioni

The sequence ABF07003.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000352 Genomic DNA. Translation: ABF07003.1. Different initiation.
RefSeqiYP_582272.3. NC_007973.1.

Genome annotation databases

EnsemblBacteriaiABF07003; ABF07003; Rmet_0117.
GeneIDi4036901.
KEGGirme:Rmet_0117.
PATRICi20284754. VBIRalMet4734_0494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000352 Genomic DNA. Translation: ABF07003.1 . Different initiation.
RefSeqi YP_582272.3. NC_007973.1.

3D structure databases

ProteinModelPortali Q1LS73.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 266264.Rmet_0117.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABF07003 ; ABF07003 ; Rmet_0117 .
GeneIDi 4036901.
KEGGi rme:Rmet_0117.
PATRICi 20284754. VBIRalMet4734_0494.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CMET266264:GJ5G-119-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CH34 / ATCC 43123 / DSM 2839.

Entry informationi

Entry nameiBIOB_RALME
AccessioniPrimary (citable) accession number: Q1LS73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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