Q1LRI7 (QUEF_RALME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	Rmet_0353

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000247715

Sequences

	Sequence		Length	Mass (Da)
	Q1LRI7 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 7575A64CD645EDED	FASTA	277	31,366
	10 20 30 40 50 60 MTLPEHSPLG KPSAYKTEYD ASLLFPIPRQ PKRAEIGLPE GRALPFFGVD IWNAYEVSWL 70 80 90 100 110 120 NLKGKPQVAL ATFIIPADTP NIVESKSFKL YLNSFNQTKI ASPEALQQLL HHDLSEATGG 130 140 150 160 170 180 TVQVRLVTEA DLGTQKMGEL DGLLLDRLDI ETDIYEPDPT LLSAEQEESP VEETLVSHLL 190 200 210 220 230 240 KSNCLVTGQP DWGSVQIRYV GAPIDQEGLL KYLISFRNHN EFHEQCVERI FTDVMRMCKP 250 260 270 VKLAVYARYT RRGGLDINPF RTNYNTPWPD NRRNARQ

References

[1]

"Complete sequence of the chromosome of Ralstonia metallidurans CH34." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000352 Genomic DNA. Translation: ABF07239.1.
RefSeq	YP_582508.1. NC_007973.1.
3D structure databases
ProteinModelPortal	Q1LRI7.
SMR	Q1LRI7. Positions 15-277.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LRI7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4037140.
GenomeReviews	Gene locus Rmet_0353 in contig CP000352_GR.
KEGG	rme:Rmet_0353.
PATRIC	20285242. VBIRalMet4734_0736.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG289139.
OMA	CVERIFC.
PhylomeDB	Q1LRI7.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	RMET266264:RMET_0353-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_RALME

Accession

Primary (citable) accession number: Q1LRI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program