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Q1LRB4 (RUVC_RALME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Rmet_0426
OrganismRalstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]
Taxonomic identifier266264 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 181181Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000002808

Sites

Metal binding71Magnesium By similarity
Metal binding671Magnesium By similarity
Metal binding1391Magnesium By similarity
Metal binding1421Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1LRB4 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: D707A6454B88A1CE

FASTA18119,020
        10         20         30         40         50         60 
MRILGIDPGL RTTGFGVLEK HGNKLAYVAS GTIRSNGETS LPERLKTLYD GISEVARTYA 

        70         80         90        100        110        120 
PDCAAIEKVF VNVNPQSTLL LGQARGAAIC GLVGQGLPVF EYTALQLKVA VVGYGRANKE 

       130        140        150        160        170        180 
QVQEMVMRLL SLSGRPSSDA ADALGVAICH ANGGDTLGTL AGLAPELARK GMRVRRGRLV 


G 

« Hide

References

[1]"Complete sequence of the chromosome of Ralstonia metallidurans CH34."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000352 Genomic DNA. Translation: ABF07312.1.
RefSeqYP_582581.1. NC_007973.1.

3D structure databases

ProteinModelPortalQ1LRB4.
SMRQ1LRB4. Positions 2-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266264.Rmet_0426.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF07312; ABF07312; Rmet_0426.
GeneID4037214.
KEGGrme:Rmet_0426.
PATRIC20285386. VBIRalMet4734_0808.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAPDCAAIE.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycCMET266264:GJ5G-442-MONOMER.

Family and domain databases

HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_RALME
AccessionPrimary (citable) accession number: Q1LRB4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families