Reviewed, UniProtKB/Swiss-Prot Q1LQG2 (HLDD_RALME)

Names and origin

Protein names

Recommended name:
    ADP-L-glycero-D-manno-heptose-6-epimerase
    EC=5.1.3.20
Alternative name(s):
    ADP-L-glycero-beta-D-manno-heptose-6-epimerase
      Short name=ADP-glyceromanno-heptose 6-epimerase
      Short name=ADP-hep 6-epimerase
      Short name=AGME

Gene names

Name:	hldD
Ordered Locus Names:	Rmet_0728

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose By similarity.
Catalytic activity	ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose. HAMAP MF_01601
Cofactor	Binds 1 NADP+ per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 4/4. HAMAP MF_01601
Subunit structure	Homopentamer By similarity.
Domain	Contains a large N-terminal NADP-binding domain, and a smaller C-terminal substrate-binding domain By similarity.
Sequence similarities	Belongs to the sugar epimerase family. HldD subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	NADP
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: HAMAP cellular metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ADP-glyceromanno-heptose 6-epimerase activity Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	ADP-L-glycero-D-manno-heptose-6-epimerase HAMAP MF_01601		PRO_0000255738
Regions
	Nucleotide binding	11 – 12	2	NADP By similarity
	Nucleotide binding	32 – 33	2	NADP By similarity
	Nucleotide binding	75 – 79	5	NADP By similarity
	Region	200 – 203	4	Substrate binding By similarity
Sites
	Active site	139	1	Proton acceptor By similarity
	Active site	177	1	Proton acceptor By similarity
	Binding site	39	1	NADP By similarity
	Binding site	54	1	NADP By similarity
	Binding site	92	1	NADP By similarity
	Binding site	143	1	NADP By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	169	1	NADP; via amide nitrogen By similarity
	Binding site	177	1	NADP By similarity
	Binding site	179	1	Substrate; via carbonyl oxygen By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	292	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LQG2-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 7D72B08B70E48514	FASTA	331	37,354
	10 20 30 40 50 60 MTIIVTGAAG FIGSNIVKGL NERGETNIIA VDNLTRAEKF NNLVDCEIAD YLDKTDFVAR 70 80 90 100 110 120 FARGDFGNVR AVFHEGACSD TMETDGRYMM ENNYRYTLAL LEACLEQGAQ FLYASSAATY 130 140 150 160 170 180 GASTMFREDR DYEKPLNVYG YSKFLFDQVV RRRLPSAHSQ IVGFRYFNVY GPREFHKGRM 190 200 210 220 230 240 ASVAFHHFNQ FRAEGTVKLF GEYNGYAPGT QSRDFVSVED VVKVNLYFLD HPEKSGIFNL 250 260 270 280 290 300 GTGRSQPFND IAVSVVNALR ESEGKPALSL DEMVQEGLVE YVKFPDALRG KYQCFTQSDV 310 320 330 SRLRGAGYSD QFLTVQEGVS RYCKWLLERS A

References

[1]

"Complete sequence of the chromosome of Ralstonia metallidurans CH34." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000352 Genomic DNA. Translation: ABF07614.1.
RefSeq	YP_582883.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4037519.
GenomeReviews	Gene locus Rmet_0728 in contig CP000352_GR.
KEGG	rme:Rmet_0728.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1LQG2.
OMA	Q1LQG2. FGPNEYH.
Enzyme and pathway databases
BioCyc	RMET266264:RMET_0728-MON.
Family and domain databases
HAMAP	MF_01601. [Tree]
InterPro	IPR001509. Epimerase_deHydtase. IPR011912. Heptose_epim. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10366:SF29. Heptose_epim. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02197. heptose_epim. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HLDD_RALME

Accession

Primary (citable) accession number: Q1LQG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)