Q1LQ72 (SYQ_RALME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	Rmet_0818

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus metallidurans

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_1000095506
Regions
	Motif	50 – 60	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	296 – 300	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	299	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LQ72 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 688FBD9447623404	FASTA	582	66,121
	10 20 30 40 50 60 MSHDTKPNDT PAASNFLRSI IDQDLATGTY AGRADKQGDP LPTVITRFPP EPNGYLHIGH 70 80 90 100 110 120 AKSICVNFGL ARDYAGRCHL RFDDTNPVKE DTEYVDSIID AVHWLGFSWD SAQAGSTPHL 130 140 150 160 170 180 YFASDYFDQL YKFAETLIER GVAYVDSQSA EQIAAMRGNF SEPGKPSPFR DRSVEENLKL 190 200 210 220 230 240 FREMRDGKYA DGEHVLRAKI DMTAPNIVMR DPVLYRIRHA HHHRTGDKWC IYPMYDFTHC 250 260 270 280 290 300 ISDAIENITH SLCTLEFENN RPLYDWVLEH LRDAGVFANP LPHQYEFARL NLTYAITSKR 310 320 330 340 350 360 RLKQLVDEQR VTGWDDPRMP TIVGIRRRGY TPESIQLFCD RVGVSKADSW IDMSTLEGAV 370 380 390 400 410 420 RDDLDARAPR SVAVLDPLKL ILDNYPEGQS EECSAPVHPK QPEMGRRVFP LSRELWIERE 430 440 450 460 470 480 DFNENPPKGY FRLFPGNKVR LRYGYVIECT GVDKDADGNV IAVHANYLPE TKSGTPGADS 490 500 510 520 530 540 VKVKGNIHWV SAPHACEAEV RLYDRLFNDP NPDAGGKNFL DALNPESKKV VTAYLEPGLR 550 560 570 580 DAKPEDRFQF ERHGYFVADR VDSQPGKPVF NRTVGLKDSW GK

References

[1]

"Complete sequence of the chromosome of Ralstonia metallidurans CH34." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000352 Genomic DNA. Translation: ABF07704.1.
RefSeq	YP_582973.1. NC_007973.1.
3D structure databases
ProteinModelPortal	Q1LQ72.
SMR	Q1LQ72. Positions 14-579.
ModBase	Search...
Protein-protein interaction databases
STRING	266264.Rmet_0818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABF07704; ABF07704; Rmet_0818.
GeneID	4037609.
KEGG	rme:Rmet_0818.
PATRIC	20286178. VBIRalMet4734_1202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	CMET266264:GJ5G-849-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_RALME

Accession

Primary (citable) accession number: Q1LQ72

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 30, 2006
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program