Q1LPX7 (ILVC_RALME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacyl reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Rmet_0913

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus metallidurans

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP-Rule MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP-Rule MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP-Rule MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP-Rule MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ketol-acid reductoisomerase HAMAP-Rule MF_00435		PRO_0000252775
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1LPX7 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: D63E49A87A265C40	FASTA	338	36,444
	10 20 30 40 50 60 MKVFYDKDAD LSLIKGKNVT IIGYGSQGHA HALNLKDSGV NVTVGLRKSG ASWNKAVNAG 70 80 90 100 110 120 LQVKEVAEAV KGADVVMILL PDEQIADVYK NEVHANIKEG AALAFAHGFN VHYGAVIPRA 130 140 150 160 170 180 DLDVIMIAPK APGHTVRSTY AQGGGVPHLI AVHQNKSGAA RDIALSYATA NGGGRAGIIE 190 200 210 220 230 240 TNFREETETD LFGEQAVLCG GTVELIKAGF ETLVEAGYAP EMAYFECLHE LKLIVDLIYE 250 260 270 280 290 300 GGIANMNYSI SNNAEYGEYV TGPRVVTEET KKAMKQCLKD IQTGEYAKSF LLENKAGAPT 310 320 330 LISRRRLTAE HQIEEVGGKL RAMMPWIAKN KLVDQSKN

References

[1]

"Complete sequence of the chromosome of Ralstonia metallidurans CH34." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000352 Genomic DNA. Translation: ABF07799.1.
RefSeq	YP_583068.1. NC_007973.1.
3D structure databases
ProteinModelPortal	Q1LPX7.
SMR	Q1LPX7. Positions 1-327.
ModBase	Search...
Protein-protein interaction databases
STRING	266264.Rmet_0913.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABF07799; ABF07799; Rmet_0913.
GeneID	4037709.
KEGG	rme:Rmet_0913.
PATRIC	20286390. VBIRalMet4734_1304.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
HOGENOM	HOG000016230.
KO	K00053.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	CMET266264:GJ5G-954-MONOMER.
UniPathway	UPA00047; UER00056. UPA00049; UER00060.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit.
HAMAP	MF_00435. IlvC.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
PANTHER	PTHR21371. PTHR21371. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_RALME

Accession

Primary (citable) accession number: Q1LPX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	May 30, 2006
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program