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Protein

Enolase 2

Gene

eno2

Organism
Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk2), Phosphoglycerate kinase (pgk1)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase 2 (eno2), Enolase 1 (eno1)
  5. Pyruvate kinase (pykA2), Pyruvate kinase (pyk), Pyruvate kinase (pykA1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155SubstrateUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Active sitei205Proton donorUniRule annotation1
Metal bindingi242MagnesiumUniRule annotation1
Metal bindingi285MagnesiumUniRule annotation1
Binding sitei285SubstrateUniRule annotation1
Metal bindingi312MagnesiumUniRule annotation1
Binding sitei312SubstrateUniRule annotation1
Active sitei337Proton acceptorUniRule annotation1
Binding sitei337Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei388SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 2UniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 2UniRule annotation
2-phosphoglycerate dehydratase 2UniRule annotation
Gene namesi
Name:eno2UniRule annotation
Ordered Locus Names:Rmet_1176
OrganismiCupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans)
Taxonomic identifieri266264 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002670851 – 425Enolase 2Add BLAST425

Proteomic databases

PRIDEiQ1LP64

Interactioni

Protein-protein interaction databases

STRINGi266264.Rmet_1176

Structurei

3D structure databases

ProteinModelPortaliQ1LP64
SMRiQ1LP64
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni364 – 367Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
HOGENOMiHOG000072174
KOiK01689
OMAiQEFLVVP
OrthoDBiPOG091H02DK

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit

Sequencei

Sequence statusi: Complete.

Q1LP64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVAEIRGL EVLDSRGNPT VEVEVVLDDG AVGRAIVPSG ASTGAREAVE
60 70 80 90 100
LRDADPRRYL GRGVLRAVQA VNTELCEALL GRDAGDQPEI DTIMIDLDGT
110 120 130 140 150
PDKRRLGANA LLGVSLAVAH AAAMSNGLPL FAYLGGERAS LLPVPLINVI
160 170 180 190 200
NGGAHADNAL DFQEFMIVPA GAPTFTEAVR ASAEVFHTLR AMLKAAGYTT
210 220 230 240 250
NVGDEGGFAP EFHAAEEALD ILVAAIAKAG YRPGEDIALA IDPAASELYV
260 270 280 290 300
NGSYVYQGEG VERSREEQVA YLVRLADRYP IVSIEDGMAE DDAMGWQLLT
310 320 330 340 350
RQLGKRCQLV GDDVFCTHPT LLRQGVEQGM ANAILVKANQ IGTLSEMRET
360 370 380 390 400
VRVAHGYAYS AVMSHRSGET EDVTIADLAV ALRCGQIKTG SMSRADRTAK
410 420
YNRLLRIERE LGSRAEYAGA LLRRR
Length:425
Mass (Da):45,532
Last modified:May 30, 2006 - v1
Checksum:i9752442518E0F051
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA Translation: ABF08062.1
RefSeqiWP_011515956.1, NC_007973.1

Genome annotation databases

EnsemblBacteriaiABF08062; ABF08062; Rmet_1176
GeneIDi24149784
KEGGirme:Rmet_1176

Similar proteinsi

Entry informationi

Entry nameiENO2_CUPMC
AccessioniPrimary (citable) accession number: Q1LP64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 30, 2006
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health