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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921S-adenosyl-L-methionineUniRule annotation
Binding sitei117 – 1171S-adenosyl-L-methionineUniRule annotation
Binding sitei144 – 1441S-adenosyl-L-methionineUniRule annotation
Active sitei167 – 1671By similarity
Binding sitei167 – 1671S-adenosyl-L-methionineUniRule annotation
Binding sitei171 – 1711SubstrateUniRule annotation
Binding sitei203 – 2031SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCMET266264:GJ5G-2886-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Rmet_2704
OrganismiRalstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Taxonomic identifieri266264 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262tRNA (guanine-N(7)-)-methyltransferasePRO_0000288208Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266264.Rmet_2704.

Structurei

3D structure databases

ProteinModelPortaliQ1LJU9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni173 – 1786Interaction with RNAUniRule annotation
Regioni241 – 2444Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1LJU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPQDTNTDP LPGDDAESAS GKSADASQGT PNPGDEVAHP RRIRSFVRRA
60 70 80 90 100
GRTSTGQQRA MDELGPTYIV PYQPEPLDWT ATFGREAPSI LEIGFGMGET
110 120 130 140 150
TAHIAGLRSQ DNFLGCEVHE PGVGALLKLI GERGLQNVRI MQHDAVEVAA
160 170 180 190 200
HMLTENSLDG VHIYFPDPWH KKRHNKRRLV QPPLVKLLAS RLKPGGYIHC
210 220 230 240 250
ATDWEEYAHQ MVEVLSGEPA LENTSKADGG FAERPDYRPV TKFEKRGLRL
260
GHGVWDVVFR KK
Length:262
Mass (Da):29,030
Last modified:May 30, 2006 - v1
Checksum:i325EF93273C5BACB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA. Translation: ABF09577.1.
RefSeqiYP_584846.1. NC_007973.1.

Genome annotation databases

EnsemblBacteriaiABF09577; ABF09577; Rmet_2704.
KEGGirme:Rmet_2704.
PATRICi20290053. VBIRalMet4734_3103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA. Translation: ABF09577.1.
RefSeqiYP_584846.1. NC_007973.1.

3D structure databases

ProteinModelPortaliQ1LJU9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266264.Rmet_2704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF09577; ABF09577; Rmet_2704.
KEGGirme:Rmet_2704.
PATRICi20290053. VBIRalMet4734_3103.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciCMET266264:GJ5G-2886-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CH34 / ATCC 43123 / DSM 2839.

Entry informationi

Entry nameiTRMB_RALME
AccessioniPrimary (citable) accession number: Q1LJU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 30, 2006
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.