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Protein

Quinolinate synthase A

Gene

nadA

Organism
Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotationSAAS annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationSAAS annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotationSAAS annotation
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit.

Pathway:iNAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei65 – 651IminoaspartateUniRule annotation
Binding sitei86 – 861Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei157 – 1571IminoaspartateUniRule annotation
Binding sitei174 – 1741IminoaspartateUniRule annotation
Binding sitei244 – 2441IminoaspartateUniRule annotation
Binding sitei261 – 2611IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseImported, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciCMET266264:GJ5G-3069-MONOMER.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotationSAAS annotation (EC:2.5.1.72UniRule annotationSAAS annotation)
Gene namesi
Name:nadAUniRule annotationImported
Ordered Locus Names:Rmet_2874Imported
OrganismiRalstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)Imported
Taxonomic identifieri266264 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi266264.Rmet_2874.

Structurei

3D structure databases

ProteinModelPortaliQ1LJC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0379.
HOGENOMiHOG000222769.
KOiK03517.
OMAiLWAPDRH.
OrthoDBiEOG6DJZ56.

Family and domain databases

HAMAPiMF_00567. NadA_type1.
InterProiIPR003473. NadA.
IPR023513. Quinolinate_synth_A_type1.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1LJC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPQTIKSVE FEKPNLTDPD AAAGGSCVAH AWAKVPPALT PDERQALKSR
60 70 80 90 100
IRKLLKERNA VLVAHYYVDA DLQDLAEETG GCVSDSLEMA RFGRDHDAKT
110 120 130 140 150
LVVAGVRFMG ETAKILSPEK TILMPDLDAT CSLDLGCPAD DFSAFCDAHP
160 170 180 190 200
DRTVVVYANT SAAVKARADW MVTSSIGLKI VEHLHARGEK ILWAPDKHLG
210 220 230 240 250
SYIQQQTGAD MLLWQGSCLV HDEFKGIELD LLRREHPKAK ILVHPESPAN
260 270 280 290 300
VVAQADVVGS TSQLIAAAQQ LDANEFIVAT DNGILHKMRM AAPGKMFIEA
310 320 330 340 350
PTAGNSATCK SCAHCPWMAM NALTNLAEVL ETGRNEIHVD ADIGRRAVTC
360 370 380
INRMLDFAAQ QKANVRPAAD LAKESALFSG VGPA
Length:384
Mass (Da):41,438
Last modified:May 30, 2006 - v1
Checksum:i9B09C4BD4C736272
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA. Translation: ABF09747.1.
RefSeqiWP_011517426.1. NC_007973.1.

Genome annotation databases

EnsemblBacteriaiABF09747; ABF09747; Rmet_2874.
GeneIDi24151720.
KEGGirme:Rmet_2874.
PATRICi20290375. VBIRalMet4734_3263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA. Translation: ABF09747.1.
RefSeqiWP_011517426.1. NC_007973.1.

3D structure databases

ProteinModelPortaliQ1LJC9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266264.Rmet_2874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF09747; ABF09747; Rmet_2874.
GeneIDi24151720.
KEGGirme:Rmet_2874.
PATRICi20290375. VBIRalMet4734_3263.

Phylogenomic databases

eggNOGiCOG0379.
HOGENOMiHOG000222769.
KOiK03517.
OMAiLWAPDRH.
OrthoDBiEOG6DJZ56.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciCMET266264:GJ5G-3069-MONOMER.

Family and domain databases

HAMAPiMF_00567. NadA_type1.
InterProiIPR003473. NadA.
IPR023513. Quinolinate_synth_A_type1.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CH34 / ATCC 43123 / DSM 2839Imported.

Entry informationi

Entry nameiQ1LJC9_RALME
AccessioniPrimary (citable) accession number: Q1LJC9
Entry historyi
Integrated into UniProtKB/TrEMBL: May 30, 2006
Last sequence update: May 30, 2006
Last modified: July 22, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.