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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei592 – 5921Aminoacyl-adenylateUniRule annotation
Binding sitei636 – 6361ATPUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi942 – 9421ZincUniRule annotation
Metal bindingi945 – 9451ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCMET266264:GJ5G-3081-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Rmet_2885
OrganismiRalstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Taxonomic identifieri266264 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000022112Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266264.Rmet_2885.

Structurei

3D structure databases

ProteinModelPortaliQ1LJB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionAdd
BLAST
Motifi633 – 6375"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1LJB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDKRAKPE KSKYPVNLLD TPFPMRGDLP KREPQWVKQW QDKQLYKKIR
60 70 80 90 100
AARHGAKKFV LHDGPPYANG DIHIGHAVNK VLKDMIIKAR GLTGLDAVYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK GLPVQEVQSK ARAYATEQIA RQRKDFERLG
160 170 180 190 200
VLGDWENPYL TMNFSNEADE LRALGKIMER GYVFRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDK VDLSIDVGFP FAETDKIAHA FHVPLAQLEG KPGWIVIWTT
260 270 280 290 300
TPWTIPSNQA LNMHPEVEYA LVDTPRGFLI LAKDRVEEQL KTYALEGTIV
310 320 330 340 350
ATARGDALTE VRFHHPLAKM DAGYVRTSPV YLGDYVTTDT GTGIVHSAPA
360 370 380 390 400
YGVEDFQSCK AHGMPDSDII SPVMGDGVYA STLPLFGGLS IWDANPKIVE
410 420 430 440 450
VLRESGNLFN SHKYEHSYMH CWRHKTPIIY RATSQWFAGM DVDPVDNGPT
460 470 480 490 500
LRETALAGIE ATEFYPAWGK QRLHNMIANR PDWTLSRQRQ WGVPMAFFVH
510 520 530 540 550
KETGALHPRT PELLEEVAKL VEKHGIEAWQ TLDPKDLLGD EAAQYEKNRD
560 570 580 590 600
TLDVWFDSGT THWTVIRGSH RDELYDPAAD LPDGRLADLY LEGSDQHRGW
610 620 630 640 650
FHSSLLTASM LYGKPPYKAL LTHGFTVDGE GRKMSKSVGN TVSPQDISNK
660 670 680 690 700
MGAEIIRLWV ASTDYSGELS ISDEILKRVV EGYRRIRNTL RFLLANLTDY
710 720 730 740 750
DHAKHALPAS EWLEIDRYAV ALTDRLQKEV LSHYQAYEFH PVVAKLQTFC
760 770 780 790 800
SEDLGGFYLD VLKDRLYTTA ADSKARRGAQ NALYHITQAM LHWMAPFLTF
810 820 830 840 850
TAEEAWQIFA HGTEHKDTIF TSTYYAIPSV DDGDTLLQKW HEIRTVRAEV
860 870 880 890 900
TRQLEAVRVE GDIGSSLQAE VTIAAGGPVL AALQSLEDDL RFVLLTSAAK
910 920 930 940 950
VTPAPEGGDL LVTVTPSQHA KCERCWHYRA DVGHNPEHPT ICGRCDSNLF

GAGEHRSHA
Length:959
Mass (Da):107,836
Last modified:May 30, 2006 - v1
Checksum:i39E25FBF00894BFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA. Translation: ABF09758.1.
RefSeqiYP_585027.1. NC_007973.1.

Genome annotation databases

EnsemblBacteriaiABF09758; ABF09758; Rmet_2885.
GeneIDi4039713.
KEGGirme:Rmet_2885.
PATRICi20290399. VBIRalMet4734_3275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA. Translation: ABF09758.1.
RefSeqiYP_585027.1. NC_007973.1.

3D structure databases

ProteinModelPortaliQ1LJB8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266264.Rmet_2885.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF09758; ABF09758; Rmet_2885.
GeneIDi4039713.
KEGGirme:Rmet_2885.
PATRICi20290399. VBIRalMet4734_3275.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCMET266264:GJ5G-3081-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CH34 / ATCC 43123 / DSM 2839.

Entry informationi

Entry nameiSYI_RALME
AccessioniPrimary (citable) accession number: Q1LJB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: January 7, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.