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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei639 – 6391ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCMET266264:GJ5G-3174-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Rmet_2973
OrganismiRalstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Taxonomic identifieri266264 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Leucine--tRNA ligasePRO_1000009406Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266264.Rmet_2973.

Structurei

3D structure databases

ProteinModelPortaliQ1LJ30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi636 – 6405"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1LJ30-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQDKYLPSAV EQAAQQHWKA IDAYKVSEHA VGPDGKEKSK FYACSMLPYP
60 70 80 90 100
SGKLHMGHVR NYTINDVMAR YLRMNGRNVL MPMGWDAFGM PAENAALNNG
110 120 130 140 150
VAPAAWTYDN IAYMKKQMQS MGLAIDWSRE VATCSPEYYR WNQWLFLKML
160 170 180 190 200
EKGIAYRKTG TVNWDPVDQT VLANEQVIDG RGWRSGAVVE KREIPMYYLR
210 220 230 240 250
ITDYAQELLG DLEGLGWPER VKIMQQNWIG RSEGVRFAFP HEIKGADGKL
260 270 280 290 300
INDGKLYVFT TRADTIMGVT FCAVAAEHPL ATHAAESNPA LAAFIEECKH
310 320 330 340 350
GSVMEADMAT MEKKGMPTGL KVTHPLTGEQ VDVWVGNYVL MTYGDGAVMG
360 370 380 390 400
VPAHDERDFA FALKYNLPIK QVIDVKGQAY STDAWLEWYG DKEHGLCIHS
410 420 430 440 450
GKYDGLGYKA AVDAIAADLA AKGLGEKKVT WRLRDWGISR QRYWGTPIPL
460 470 480 490 500
IHCDSCGVVP VPEKDLPVVL PEDLVPDGTG NPLAKDPRFL ECTCPSCGKP
510 520 530 540 550
ARRETDTMDT FIDSCWYYMR YTCPDAGTMV DARNDYWMPM DQYIGGIEHA
560 570 580 590 600
ILHLLYARFW TKVMRDMGLV KFDEPFTNLL TQGMVLNETF YREDASGKKT
610 620 630 640 650
WYNPADVDVQ TDERGRPAGA TAKADGQPVV IGGIEKMSKS KNNGIDPQAL
660 670 680 690 700
IDQYGADTAR LFVMFAAPPE QQLEWSGSGV EGASRFLRRV WNYGYANAQA
710 720 730 740 750
IRDGAGTAPT ADDAALRREI HTVLKQANYD YERIQYNTVV SATMKMLNAL
760 770 780 790 800
EDAKTASPAG RREGFSVLLR VLYPVVPHIA HGLWQELGYA AETVDILDAA
810 820 830 840 850
WPQVDEAALV RSEIELVLQV NGKVRGSLTV PADADRAAIE ATAAASEIVA
860 870
KFAAGAAPKK IVVVPGRLVN VVL
Length:873
Mass (Da):96,869
Last modified:May 30, 2006 - v1
Checksum:i5641A1642E217597
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA. Translation: ABF09846.1.
RefSeqiYP_585115.1. NC_007973.1.

Genome annotation databases

EnsemblBacteriaiABF09846; ABF09846; Rmet_2973.
GeneIDi4039801.
KEGGirme:Rmet_2973.
PATRICi20290585. VBIRalMet4734_3368.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000352 Genomic DNA. Translation: ABF09846.1.
RefSeqiYP_585115.1. NC_007973.1.

3D structure databases

ProteinModelPortaliQ1LJ30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266264.Rmet_2973.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF09846; ABF09846; Rmet_2973.
GeneIDi4039801.
KEGGirme:Rmet_2973.
PATRICi20290585. VBIRalMet4734_3368.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCMET266264:GJ5G-3174-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CH34 / ATCC 43123 / DSM 2839.

Entry informationi

Entry nameiSYL_RALME
AccessioniPrimary (citable) accession number: Q1LJ30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: January 7, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.