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Q1LJ30 (SYL_RALME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Rmet_2973
OrganismRalstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]
Taxonomic identifier266264 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length873 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 873873Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009406

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_00049
Motif636 – 6405"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6391ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1LJ30 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: 5641A1642E217597

FASTA87396,869
        10         20         30         40         50         60 
MQDKYLPSAV EQAAQQHWKA IDAYKVSEHA VGPDGKEKSK FYACSMLPYP SGKLHMGHVR 

        70         80         90        100        110        120 
NYTINDVMAR YLRMNGRNVL MPMGWDAFGM PAENAALNNG VAPAAWTYDN IAYMKKQMQS 

       130        140        150        160        170        180 
MGLAIDWSRE VATCSPEYYR WNQWLFLKML EKGIAYRKTG TVNWDPVDQT VLANEQVIDG 

       190        200        210        220        230        240 
RGWRSGAVVE KREIPMYYLR ITDYAQELLG DLEGLGWPER VKIMQQNWIG RSEGVRFAFP 

       250        260        270        280        290        300 
HEIKGADGKL INDGKLYVFT TRADTIMGVT FCAVAAEHPL ATHAAESNPA LAAFIEECKH 

       310        320        330        340        350        360 
GSVMEADMAT MEKKGMPTGL KVTHPLTGEQ VDVWVGNYVL MTYGDGAVMG VPAHDERDFA 

       370        380        390        400        410        420 
FALKYNLPIK QVIDVKGQAY STDAWLEWYG DKEHGLCIHS GKYDGLGYKA AVDAIAADLA 

       430        440        450        460        470        480 
AKGLGEKKVT WRLRDWGISR QRYWGTPIPL IHCDSCGVVP VPEKDLPVVL PEDLVPDGTG 

       490        500        510        520        530        540 
NPLAKDPRFL ECTCPSCGKP ARRETDTMDT FIDSCWYYMR YTCPDAGTMV DARNDYWMPM 

       550        560        570        580        590        600 
DQYIGGIEHA ILHLLYARFW TKVMRDMGLV KFDEPFTNLL TQGMVLNETF YREDASGKKT 

       610        620        630        640        650        660 
WYNPADVDVQ TDERGRPAGA TAKADGQPVV IGGIEKMSKS KNNGIDPQAL IDQYGADTAR 

       670        680        690        700        710        720 
LFVMFAAPPE QQLEWSGSGV EGASRFLRRV WNYGYANAQA IRDGAGTAPT ADDAALRREI 

       730        740        750        760        770        780 
HTVLKQANYD YERIQYNTVV SATMKMLNAL EDAKTASPAG RREGFSVLLR VLYPVVPHIA 

       790        800        810        820        830        840 
HGLWQELGYA AETVDILDAA WPQVDEAALV RSEIELVLQV NGKVRGSLTV PADADRAAIE 

       850        860        870 
ATAAASEIVA KFAAGAAPKK IVVVPGRLVN VVL 

« Hide

References

[1]"Complete sequence of the chromosome of Ralstonia metallidurans CH34."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000352 Genomic DNA. Translation: ABF09846.1.
RefSeqYP_585115.1. NC_007973.1.

3D structure databases

ProteinModelPortalQ1LJ30.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266264.Rmet_2973.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF09846; ABF09846; Rmet_2973.
GeneID4039801.
KEGGrme:Rmet_2973.
PATRIC20290585. VBIRalMet4734_3368.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCMET266264:GJ5G-3174-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RALME
AccessionPrimary (citable) accession number: Q1LJ30
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries