Q1LH11 (CHEB1_RALME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Rmet_3693

Encoded on

Plasmid megaplasmid CH34

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264300
Regions
	Domain	7 – 124	118	Response regulatory
	Domain	158 – 350	193	CheB-type methylesterase
Sites
	Active site	170	1	By similarity
	Active site	196	1	By similarity
	Active site	292	1	By similarity
Amino acid modifications
	Modified residue	58	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LH11 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 6E1B43BD2A897D77	FASTA	357	38,474
	10 20 30 40 50 60 MTAAKIKVLC VDDSALIRSL MSEIINSQPD MEVVGTAPDP LVARDLIKRV NPDVLTLDVE 70 80 90 100 110 120 MPRMDGLDFL ERLMRLRPMP VLMVSSLTER GSEITMRALE LGAVDFVTKP KLGIREGLLD 130 140 150 160 170 180 YTQTIADKIR AASRARVRAR AEQSAGAAPV APMLRAPLLS TEKLIIVGAS TGGTEAIKDF 190 200 210 220 230 240 LTPLPPDSPA VMIVQHMPAG FTKSFAHRLN GLCRITVKEA EHGERVLPGY AYIAPGDSHL 250 260 270 280 290 300 LLARSGANYV AHLSQEAPVN RHRPSVDVLF DSAAIHGGKN VTGVILTGMG KDGARGMLRM 310 320 330 340 350 REAGAYNLAQ DEQSCIVFGM PKEAIAAGGV HEVVPLHQMS QRVMAHLATF GARAQRV

References

[1]

"Complete sequence of the megaplasmid of Ralstonia metallidurans CH34." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000353 Genomic DNA. Translation: ABF10565.1.
RefSeq	YP_585834.1. NC_007974.2.
3D structure databases
ProteinModelPortal	Q1LH11.
SMR	Q1LH11. Positions 4-348.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LH11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4040664.
GenomeReviews	Gene locus Rmet_3693 in contig CP000353_GR.
KEGG	rme:Rmet_3693.
PATRIC	20292098. VBIRalMet4734_4100.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	FVTKPKL.
PhylomeDB	Q1LH11.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	RMET266264:RMET_3693-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_RALME

Accession

Primary (citable) accession number: Q1LH11

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program