Q1LFU5 (PDXK_RALME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyridoxine kinase
EC=2.7.1.35

Alternative name(s):
PN/PL/PM kinase
Pyridoxal kinase
Pyridoxamine kinase
Vitamin B6 kinase

Gene names

Name:	pdxK
Ordered Locus Names:	Rmet_4114

Encoded on

Plasmid megaplasmid

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates By similarity. HAMAP MF_01638
Catalytic activity	ATP + pyridoxal = ADP + pyridoxal 5'-phosphate. HAMAP MF_01638
Cofactor	Zinc or magnesium By similarity. HAMAP MF_01638
Sequence similarities	Belongs to the pyridoxine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	pyridoxal 5'-phosphate salvage Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Pyridoxine kinase HAMAP MF_01638		PRO_0000268839
Regions
	Nucleotide binding	195 – 196	2	ATP By similarity
Sites
	Binding site	23	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	234	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1LFU5 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: C2410E0E277F9EA2	FASTA	288	30,799
	10 20 30 40 50 60 MFGETGTGDA LRPIFMDVVS VQSQVVYGHV GNNVALPTLR AHGLNVAAVP TVMFSNTPHY 70 80 90 100 110 120 PTLHGGALPI DWFGGYLSDL SARGALQRLK AILVGYLGNP EQVAVLSRWI NQVLAEHPDV 130 140 150 160 170 180 QVIVDPVIGD HDTGIYVADG MVEAVRELLL PLAHGLTPND FELGHLSGRS ADSVEQVVAA 190 200 210 220 230 240 ARSLLTDRVQ WMVVTSAAPG TWGHDDMKLL IVTRDDAQVL SHPRIPVSPK GTGDLFSAKL 250 260 270 280 TARLLEGMPL AEAAASASDH VVTALEATRR AQSLELQLPS NPCITHRE

References

[1]

"Complete sequence of the megaplasmid of Ralstonia metallidurans CH34." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000353 Genomic DNA. Translation: ABF10981.1.
RefSeq	YP_586250.1. NC_007974.2.
3D structure databases
ProteinModelPortal	Q1LFU5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LFU5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4040972.
GenomeReviews	Gene locus Rmet_4114 in contig CP000353_GR.
KEGG	rme:Rmet_4114.
PATRIC	20292954. VBIRalMet4734_4522.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2240.
HOGENOM	HBG661459.
OMA	RARMLVS.
PhylomeDB	Q1LFU5.
ProtClustDB	PRK08176.
Enzyme and pathway databases
BioCyc	RMET266264:RMET_4114-MONOMER.
Family and domain databases
HAMAP	MF_01638. PdxK. [Tree]
InterPro	IPR013749. HMP-P_kinase-1. IPR004625. PyrdxlP_synth_PyrdxlKinase. IPR023479. Pyridoxal_kinase_PdxK. [Graphical view]
KO	K00868.
PANTHER	PTHR10534. PTHR10534. 1 hit.
Pfam	PF08543. Phos_pyr_kin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00687. Pyridox_kin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXK_RALME

Accession

Primary (citable) accession number: Q1LFU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program