Q1LFS9 (Q1LFS9_RALME) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase HAMAP MF_01902
EC=2.7.7.7 HAMAP MF_01902

Gene names

Name:	dnaE2 HAMAP MF_01902 EMBL ABF10997.1
Ordered Locus Names:	Rmet_4130

Encoded on

Plasmid megaplasmid CH34

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	1153 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902 SAAS SAAS004013
Subcellular location	Cytoplasm By similarity HAMAP MF_01902 SAAS SAAS004013.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily. HAMAP MF_01902

Ontologies

Keywords
Biological process	DNA damage DNA repair HAMAP MF_01902 SAAS SAAS004013 DNA replication HAMAP MF_01902 SAAS SAAS004013
Cellular component	Cytoplasm HAMAP MF_01902 SAAS SAAS004013
Molecular function	DNA-directed DNA polymerase HAMAP MF_01902 SAAS SAAS004013 Nucleotidyltransferase Transferase
Technical term	Complete proteome Plasmid EMBL ABF10997.1
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q1LFS9 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: D5CA443CD3353632	FASTA	1,153	128,615
	10 20 30 40 50 60 MLSGLSSLLP SLPDYVELHC ISNFTFLNGA SHPQELISRA FQLGYSGLAL TDECSVAGTA 70 80 90 100 110 120 RAHHAIKVLR EDMWEAVTRS ARRLAAGQTA GDVDEIEELS AFDLDTGGEE SDAGGVDDAE 130 140 150 160 170 180 DTEDEDALSP DDFDDDPDAA ARRHRVLEAR AAAADAFNLI IGSRFTLTAA PEDTMPPPRL 190 200 210 220 230 240 VLLARNRIGF GDLCEFITLG RTRAEKGSYL LYPSDLADPQ PEQAHLRGMP DCLVVLLPDY 250 260 270 280 290 300 CADLDVLRAQ ARWCRATFGD RAWMAMELLQ GHAEAMHRAR LQQVSAETGV PLVAAGAVNM 310 320 330 340 350 360 HVRSRKPLAD VLTAIRIGQP LSACGRALAP NAEAHLRMRQ VLSRLYPREA LAQTLKLADQ 370 380 390 400 410 420 CRFSLDMLRY EYPEELVPDG ETPITYLRKE VERGITERFP HGMAPEWKKQ LKEELELIEE 430 440 450 460 470 480 KHYEPFFLTV HDIVRFARQE GILCQGRGSA ANSLVCYCLH ITEVSPEQAN LLFGRFLSRE 490 500 510 520 530 540 RDEPPDIDVD FEHQRREEVI QYIYGKYGRH RAALAASLIT YRSRSALRDV GRALGIDPGV 550 560 570 580 590 600 VEQVVKGQAW WDGGAAFIDR ISRYGLDPDS ITVRQWASLT EQLRGFPRHL SQHVGGFVIA 610 620 630 640 650 660 RDKLSRLVPI ENAAMENRSV IQWDKDDLES LGLLKVDVLA LGMLTAIRRA LTMIPNPDPE 670 680 690 700 710 720 RAGLPTRMED LPDGDVKTYD MICKADTIGV FQIESRAQQS MLPRLQPREY YDLVVEVAIV 730 740 750 760 770 780 RPGPIQGGMV HPYLKRREAF RLTRKLPTYF DDVIRRVLGR TLGVPIFQEQ VMQLAIEAAG 790 800 810 820 830 840 FTPGQADQLR RSMAAWRRRG DLKKHQDALV RALTGRGYPE SFALAICKQI EGFGEYGFPE 850 860 870 880 890 900 SHAASFAKLV YVSAWIKCHY PEAFLCALLN SQPMGFYSPS QLVQDARRHG VTSQPVDVTI 910 920 930 940 950 960 SNWESTLEPR REGQNFRDVR LGMNRVKGMR EAAALRIEAA RVARPFDSVE DLARRASLDR 970 980 990 1000 1010 1020 HDIDALAAAD ALVSLAGHRR QARWHARGAA VQTAHRDLLH DAPPQEHALQ LPEPKLGEDV 1030 1040 1050 1060 1070 1080 AADYASLGLS LKCHPLTLLR HRLRALKFAT ARQLANCANG RRVRACGIVT VRQRPSTASG 1090 1100 1110 1120 1130 1140 TIFTSIEDET GAVNVIIWPD LVERQRKEVL GATLLGVIGT WQRQGEVRHL VADELVDLSP 1150 LLGRLMVGSR DFH

References

[1]

"Complete sequence of the megaplasmid of Ralstonia metallidurans CH34." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CH34 / ATCC 43123 / DSM 2839.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000353 Genomic DNA. Translation: ABF10997.1.
RefSeq	YP_586266.1. NC_007974.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1LFS9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4040988.
GenomeReviews	Gene locus Rmet_4130 in contig CP000353_GR.
KEGG	rme:Rmet_4130.
PATRIC	20292988. VBIRalMet4734_4539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	LATNRNG.
ProtClustDB	PRK05672.
Enzyme and pathway databases
BioCyc	RMET266264:RMET_4130-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q1LFS9_RALME

Accession

Primary (citable) accession number: Q1LFS9

Entry history

Integrated into UniProtKB/TrEMBL:	May 30, 2006
Last sequence update:	May 30, 2006
Last modified:	December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)