Q1L8L9 (KAD2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate kinase 2, mitochondrial
Short name=AK 2
EC=2.7.4.3

Alternative name(s):
ATP-AMP transphosphorylase 2

Gene names

Name:	ak2
ORF Names:	si:ch211-197n10.1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis. Ref.3
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion intermembrane space By similarity.
Disruption phenotype	Leads to aberrant leukocyte development. Ref.3
Sequence similarities	Belongs to the adenylate kinase family. AK2 subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	leukocyte differentiation Inferred from mutant phenotype Ref.3. Source: ZFIN
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Adenylate kinase 2, mitochondrial		PRO_0000365695
Regions
	Nucleotide binding	24 – 32	9	ATP By similarity
	Nucleotide binding	47 – 76	30	AMP By similarity
Sites
	Binding site	48	1	AMP By similarity
Amino acid modifications
	Disulfide bond	44 ↔ 94		By similarity
Experimental info
	Sequence conflict	93	1	A → S in AAH53160. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q1L8L9 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: D0AA5E0D3F374E4E	FASTA	241	26,616
	10 20 30 40 50 60 MAPSTQEDDT VSGIRKGIRA ILLGPPGAGK GTQAPKLAEK YCVCHLATGD MLRAMVASGS 70 80 90 100 110 120 ELGQRLKETM DAGKLVSDEM VVELIDNNLD TPACKNGFLL DGFPRTVKQA EMLDDLMEKR 130 140 150 160 170 180 SEKLDSVIEF SVDDSLLVRR ICGRLIHQPS GRSYHEEFHP PKEHMKDDVT GEPLIRRSDD 190 200 210 220 230 240 NETTLRSRLE SYHRQTSPLV QYYSARGLHT AIDASQSTDL VFASILAAFS AATCKDLVYF V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Reticular dysgenesis (aleukocytosis) is caused by mutations in the gene encoding mitochondrial adenylate kinase 2." Pannicke U., Hoenig M., Hess I., Friesen C., Holzmann K., Rump E.-M., Barth T.F., Rojewski M.T., Schulz A., Boehm T., Friedrich W., Schwarz K. Nat. Genet. 41:101-105(2009) [PubMed: 19043417] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX957241, CR753876 Genomic DNA. Translation: CAK04357.1. CR753876, BX957241 Genomic DNA. Translation: CAK11314.1. BC053160 mRNA. Translation: AAH53160.1.
IPI	IPI00505523.
RefSeq	NP_997761.1. NM_212596.1.
UniGene	Dr.61277.
3D structure databases
ProteinModelPortal	Q1L8L9.
SMR	Q1L8L9. Positions 17-234.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1L8L9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000003167; ENSDARP00000010686; ENSDARG00000005926.
GeneID	321793.
KEGG	dre:321793.
NMPDR	fig|7955.3.peg.10554.
Organism-specific databases
CTD	204.
ZFIN	ZDB-GENE-030131-512. ak2.
Phylogenomic databases
eggNOG	fiNOG13543.
GeneTree	ENSGT00600000084421.
HOGENOM	HBG630208.
HOVERGEN	HBG000458.
InParanoid	Q1L8L9.
OMA	HEEFHPP.
OrthoDB	EOG483D5C.
PhylomeDB	Q1L8L9.
Gene expression databases
ArrayExpress	Q1L8L9.
Bgee	Q1L8L9.
Family and domain databases
InterPro	IPR006259. Adenyl_kin_sub. IPR000850. Adenylate_kin. IPR007862. Adenylate_kinase_lid-dom. [Graphical view]
KO	K00939.
PANTHER	PTHR23359. Adenylate_kin. 1 hit.
Pfam	PF00406. ADK. 1 hit. PF05191. ADK_lid. 1 hit. [Graphical view]
PRINTS	PR00094. ADENYLTKNASE.
TIGRFAMs	TIGR01351. Adk. 1 hit.
PROSITE	PS00113. ADENYLATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAD2_DANRE

Accession

Primary (citable) accession number: Q1L8L9
Secondary accession number(s): Q7T3D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	May 30, 2006
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program