Q1L6Q1 (DUSTY_APIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual serine/threonine and tyrosine protein kinase
EC=2.7.12.1

Alternative name(s):
Dusty protein kinase
Short name=Dusty PK
Receptor-interacting serine/threonine-protein kinase 5

Gene names

Name:	DSTYK
Synonyms:	RIPK5

Organism

Apis mellifera (Honeybee)

Taxonomic identifier

7460 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Apoidea › Apidae › Apis

Protein attributes

Sequence length	969 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB P16879
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q1L6Q1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q1L6Q1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.
     39-41: FPE → MFT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 969	969	Dual serine/threonine and tyrosine protein kinase		PRO_0000374060
Regions
	Domain	632 – 894	263	Protein kinase
	Nucleotide binding	638 – 646	9	ATP By similarity
	Coiled coil	7 – 37	31	Potential
Sites
	Active site	760	1	Proton acceptor By similarity
	Binding site	662	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 38	38	Missing in isoform 2. Ref.1		VSP_053089
	Alternative sequence	39 – 41	3	FPE → MFT in isoform 2. Ref.1		VSP_053090

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: CBE73908BE56C06D	FASTA	969	110,428
	10 20 30 40 50 60 MVSKLPQEFR RYLRNRNQLQ HVLEETQQAL ELINLENFFP EENVVEELLS PLTLNRITHI 70 80 90 100 110 120 LANSPAIIIL GQDSKAKAIV VNTLISNDIL PVCNGLWRWI RLTYGQTNHI SLTLDLEYEL 130 140 150 160 170 180 VENLQANEKP WSTLPIEDLT KSDNEDITYP TVLEVQLNLP ILKDGVQIFI APNNGAVKVL 190 200 210 220 230 240 ANEFLSILPI FLYALGEQPL TEQNLEELRD LKETYPFNPV LFISSLENIS LNGIDPELTE 250 260 270 280 290 300 SEQHRLQNRL YTNDSTSSKT DDDSIDFDRM NSLGLSWLDQ LTNLGFLGMK ESVEVDQLSW 310 320 330 340 350 360 LGSGQYISDF VGSCRKTDQI LYFIRGCLQT YLINASTYLN EVHTASLRKF ILSAFDMARI 370 380 390 400 410 420 IQITPKRIQY AQHKENELYA NLMKIVHEKQ QEITGLIQNI IQEMKNDVLL SNNDVYLYQN 430 440 450 460 470 480 TMSNNNQRTE WSATVKAAIS EVQRVVLGRL CEKVAKQLVN SVNCLRESFI GTLQRCLLSL 490 500 510 520 530 540 EKTYERDTCL LASDALKQIL SAAYNVELHN SSPFSIYSFL ERLNLIFMSS SLQWSSTHTL 550 560 570 580 590 600 DVAWRRKVTI EILNSLSATK LSKIILMQFG DKLESSHDSF QAALRSIENY YSGKLERTEE 610 620 630 640 650 660 QRIALRKYHA PRLAKLALES TSMIDVVRYG KPHCAEEIGR GQYGIVFACD GWGGKAGPCA 670 680 690 700 710 720 IKSVVPSDES HWNDLAMEFY YNRSIPDHKR IVKLRGSIID HSYGGGFGFG SAVLLISDRL 730 740 750 760 770 780 SRDLYCGIRA GLSWLERIQI ALDVLEGIRY LHSQGLVHRD VKLKNVLLDI ENRAKLTDFG 790 800 810 820 830 840 FCITEVMMLG SIVGTPVHMA PELLSGHYDS SVDVYAFGIL FWYLCAGHVR LPYTFEQFHN 850 860 870 880 890 900 KELLWTSVKK ALMIVGIRPE RLPSFDDECW RLMEQCWSGE PSKRPLLGAI VPVLESIQQK 910 920 930 940 950 960 AKRSKSLQEV SSDKLQESST DSRNPALALA EPYNQRGTVV SPPPTKRRTM KVVKYHLFLT NLYIQMREL
	Isoform 2 [UniParc]. Checksum: 63D2B726D8DA9983	FASTA	931	105,752
	10 20 30 40 50 60 MFTENVVEEL LSPLTLNRIT HILANSPAII ILGQDSKAKA IVVNTLISND ILPVCNGLWR 70 80 90 100 110 120 WIRLTYGQTN HISLTLDLEY ELVENLQANE KPWSTLPIED LTKSDNEDIT YPTVLEVQLN 130 140 150 160 170 180 LPILKDGVQI FIAPNNGAVK VLANEFLSIL PIFLYALGEQ PLTEQNLEEL RDLKETYPFN 190 200 210 220 230 240 PVLFISSLEN ISLNGIDPEL TESEQHRLQN RLYTNDSTSS KTDDDSIDFD RMNSLGLSWL 250 260 270 280 290 300 DQLTNLGFLG MKESVEVDQL SWLGSGQYIS DFVGSCRKTD QILYFIRGCL QTYLINASTY 310 320 330 340 350 360 LNEVHTASLR KFILSAFDMA RIIQITPKRI QYAQHKENEL YANLMKIVHE KQQEITGLIQ 370 380 390 400 410 420 NIIQEMKNDV LLSNNDVYLY QNTMSNNNQR TEWSATVKAA ISEVQRVVLG RLCEKVAKQL 430 440 450 460 470 480 VNSVNCLRES FIGTLQRCLL SLEKTYERDT CLLASDALKQ ILSAAYNVEL HNSSPFSIYS 490 500 510 520 530 540 FLERLNLIFM SSSLQWSSTH TLDVAWRRKV TIEILNSLSA TKLSKIILMQ FGDKLESSHD 550 560 570 580 590 600 SFQAALRSIE NYYSGKLERT EEQRIALRKY HAPRLAKLAL ESTSMIDVVR YGKPHCAEEI 610 620 630 640 650 660 GRGQYGIVFA CDGWGGKAGP CAIKSVVPSD ESHWNDLAME FYYNRSIPDH KRIVKLRGSI 670 680 690 700 710 720 IDHSYGGGFG FGSAVLLISD RLSRDLYCGI RAGLSWLERI QIALDVLEGI RYLHSQGLVH 730 740 750 760 770 780 RDVKLKNVLL DIENRAKLTD FGFCITEVMM LGSIVGTPVH MAPELLSGHY DSSVDVYAFG 790 800 810 820 830 840 ILFWYLCAGH VRLPYTFEQF HNKELLWTSV KKALMIVGIR PERLPSFDDE CWRLMEQCWS 850 860 870 880 890 900 GEPSKRPLLG AIVPVLESIQ QKAKRSKSLQ EVSSDKLQES STDSRNPALA LAEPYNQRGT 910 920 930 VVSPPPTKRR TMKVVKYHLF LTNLYIQMRE L

References

[1]

"Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain." Peng J., Dong W., Chen Y., Mo R., Cheng J.-F., Hui C.-C., Mohandas N., Huang C.-H. Biochim. Biophys. Acta 1759:562-572(2006) [PubMed: 17123648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ013067 mRNA. Translation: AAY81955.1. DQ013068 mRNA. Translation: AAY81956.1.
RefSeq	NP_001071281.1. NM_001077813.1.
UniGene	Ame.8046.
3D structure databases
ProteinModelPortal	Q1L6Q1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	412747.
KEGG	ame:412747.
Organism-specific databases
CTD	412747.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
KO	K08282.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUSTY_APIME

Accession

Primary (citable) accession number: Q1L6Q1
Secondary accession number(s): Q1L6Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 30, 2006
Last modified:	November 16, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)