ID   PYLS_METTE              Reviewed;         478 AA.
AC   Q1L6A3;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 17.
DE   RecName: Full=Pyrrolysyl-tRNA synthetase;
DE            EC=6.1.1.26;
DE   AltName: Full=Pyrrolysine--tRNA ligase;
DE            Short=PylRS;
GN   Name=pylS;
OS   Methanosarcina thermophila.
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2210;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Polycarpo C., Ambrogelly A., Herring S., Soell D.G.;
RT   "The substrate specificity of pyrrolysyl-tRNA synthetase.";
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the attachment of pyrrolysine to tRNA(Pyl).
CC       Pyrrolysine is a lysine derivative encoded by the termination
CC       codon UAG (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + L-pyrrolysine + tRNA(Pyl) = AMP +
CC       diphosphate + L-pyrrolysyl-tRNA(Pyl).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; DQ017250; AAY81923.1; -; Genomic_DNA.
DR   BRENDA; 6.1.1.26; 8918.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004812; F:aminoacyl-tRNA ligase activity; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0006418; P:tRNA aminoacylation for protein translation; IEA:HAMAP.
DR   HAMAP; MF_01573; -; 1.
DR   InterPro; IPR018150; aa-tRNA-synt_II-like.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb_cons-reg.
DR   InterPro; IPR006195; aa-tRNA-synth_II_cons-reg.
DR   InterPro; IPR012739; PylS.
DR   PANTHER; PTHR22594; aa-tRNA-synt_II; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   TIGRFAMs; TIGR02367; PylS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN         1    478       Pyrrolysyl-tRNA synthetase.
FT                                /FTId=PRO_0000260454.
SQ   SEQUENCE   478 AA;  53441 MW;  348D3824845E0278 CRC64;
     MDKKPLNTLI SATGLWMSRT GKLHKIRHHE VSKRKIYIEM ECGERLVVNN SRSCRAARAL
     RHHKYRKICK HCRVSDEDLN KFLTRTNEDK SNAKVTVVSA PKIRKVMPKS VARTPKPLEN
     TAPVQTLPSE SQPAPTTPIS ASTTAPASTS TTAPAPASTT APAPASTTAP ASASTTISTS
     AMPASTSAQG TTKFNYISGG FPRPIPVQAS APALTKSQID RLQGLLSPKD EISLDSGTPF
     RKLESELLSR RRKDLKQIYA EEREHYLGKL EREITKFFVD RGFLEIKSPI LIPMEYIERM
     GIDNDKELSK QIFRVDNNFC LRPMLAPNLY NYLRKLNRAL PDPIKIFEIG PCYRKESDGK
     EHLEEFTMLN FCQMGSGCTR ENLEAIIKDF LDYLGIDFEI VGDSCMVYGD TLDVMHGDLE
     LSSAVVGPVP MDRDWGINKP WIGAGFGLER LLKVMHNFKN IKRASRSESY YNGISTNL
//