Reviewed, UniProtKB/Swiss-Prot Q1L6A3 (PYLS_METTE)

Names and origin

Protein names

Recommended name:
    Pyrrolysyl-tRNA synthetase
    EC=6.1.1.26
Alternative name(s):
    Pyrrolysine--tRNA ligase
      Short name=PylRS

Gene names

Name:

pylS

Organism

Methanosarcina thermophila

Taxonomic identifier

2210 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG By similarity.
Catalytic activity	ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl). HAMAP MF_01573
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Pyrrolysyl-tRNA synthetase HAMAP MF_01573		PRO_0000260454

Sequences

	Sequence		Length	Mass (Da)
	Q1L6A3-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 348D3824845E0278	FASTA	478	53,441
	10 20 30 40 50 60 MDKKPLNTLI SATGLWMSRT GKLHKIRHHE VSKRKIYIEM ECGERLVVNN SRSCRAARAL 70 80 90 100 110 120 RHHKYRKICK HCRVSDEDLN KFLTRTNEDK SNAKVTVVSA PKIRKVMPKS VARTPKPLEN 130 140 150 160 170 180 TAPVQTLPSE SQPAPTTPIS ASTTAPASTS TTAPAPASTT APAPASTTAP ASASTTISTS 190 200 210 220 230 240 AMPASTSAQG TTKFNYISGG FPRPIPVQAS APALTKSQID RLQGLLSPKD EISLDSGTPF 250 260 270 280 290 300 RKLESELLSR RRKDLKQIYA EEREHYLGKL EREITKFFVD RGFLEIKSPI LIPMEYIERM 310 320 330 340 350 360 GIDNDKELSK QIFRVDNNFC LRPMLAPNLY NYLRKLNRAL PDPIKIFEIG PCYRKESDGK 370 380 390 400 410 420 EHLEEFTMLN FCQMGSGCTR ENLEAIIKDF LDYLGIDFEI VGDSCMVYGD TLDVMHGDLE 430 440 450 460 470 LSSAVVGPVP MDRDWGINKP WIGAGFGLER LLKVMHNFKN IKRASRSESY YNGISTNL

References

[1]	"The substrate specificity of pyrrolysyl-tRNA synthetase." Polycarpo C., Ambrogelly A., Herring S., Soell D.G. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ017250 Genomic DNA. Translation: AAY81923.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.1.1.26. 8918.
Family and domain databases
HAMAP	MF_01573. [Tree]
InterPro	IPR018150. aa-tRNA-synt_II-like. IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR012739. PylS. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
TIGRFAMs	TIGR02367. PylS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYLS_METTE

Accession

Primary (citable) accession number: Q1L6A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)