Reviewed, UniProtKB/Swiss-Prot Q1L5Z8 (HOG1_TORDE)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase HOG1
      Short name=MAP kinase HOG1
    EC=2.7.11.24

Gene names

Name:

HOG1

Organism

Torulaspora delbrueckii (Yeast) (Saccharomyces rosei)

Taxonomic identifier

4950 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Torulaspora

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes By similarity. Involved in resistence to osmotic stress and tolerance to methylglyoxal and citric acid.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-174 and Tyr-176, which activates the enzyme By similarity. Phosphorylated during osmotic stress.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Mitogen-activated protein kinase HOG1		PRO_0000289702
Regions
	Domain	23 – 302	280	Protein kinase
	Nucleotide binding	29 – 37	9	ATP By similarity
	Motif	174 – 176	3	TXY
	Compositional bias	364 – 383	20	Poly-Ala
Sites
	Active site	144	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity
Amino acid modifications
	Modified residue	174	1	Phosphothreonine By similarity
	Modified residue	176	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1L5Z8-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: AC6BFC70A4D50D98	FASTA	427	48,021
	10 20 30 40 50 60 MATHEEFIRT QVFGTVFEIT NRYTDLNPVG MGAFGLVCSA TDTLAQQPVA IKKIMKPFST 70 80 90 100 110 120 AVLAKRTYRE LKLLKHLRHE NLICLQDIFL SPLEDIYFVT ELQGTDLHRL LQTRPLEKQF 130 140 150 160 170 180 VQYFLYQILR GLKYVHSAGV IHRDLKPSNI LINENCDLKI CDFGLARIQD PQMTGYVSTR 190 200 210 220 230 240 YYRAPEIMLT WQKYDVEVDI WSAGCIFAEM TEGKPLFPGK DHVHQFSIIT DLLGSPPEDV 250 260 270 280 290 300 INTICSENTL KFVTSLPHRD PVPFRERFKT VEPEAVDLLE KMLVFDPKKR ITAADALVHP 310 320 330 340 350 360 YLAPYHDPTD EPVADAKFDW NFNDADLPVD TWRVMMYSEI LDFHKIGGGD GQIDTSATFD 370 380 390 400 410 420 DQVAAATAAA AHAAAVAQAQ AQAHSNSSNS NSSSNVNSKS KAARDSANDA ITNYGNQAVH YANEFQQ

References

[1]

"Hog1 mitogen-activated protein kinase plays conserved and distinct roles in the osmotolerant yeast Torulaspora delbrueckii." Hernandez-Lopez M.J., Randez-Gil F., Prieto J.A. Eukaryot. Cell 5:1410-1419(2006) [PubMed: 16896224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION. Strain: PYCC 5321 / CECT 11342.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ020519 Genomic DNA. Translation: AAY84830.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 264341.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_TORDE

Accession

Primary (citable) accession number: Q1L5Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 30, 2006
Last modified:	September 22, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)