Reviewed, UniProtKB/Swiss-Prot Q1KZG4 (FHIT_BOVIN)

Names and origin

Protein names

Recommended name:
    Bis(5'-adenosyl)-triphosphatase
    EC=3.6.1.29
Alternative name(s):
    Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
    Dinucleosidetriphosphatase
    AP3A hydrolase
      Short name=AP3Aase
    Fragile histidine triad protein

Gene names

Name:

FHIT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves A-5'-PPP-5'A to yield AMP and ADP By similarity.
Catalytic activity	P(1)-P(3)-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP.
Cofactor	Divalent cations. Magnesium, but manganese and to a lesser extent calcium or cobalt can be substituted; but not zinc, cadmium or nickel By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in the brain, kidney, spleen, testis and lung. Ref.2
Sequence similarities	Contains 1 HIT domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Manganese
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	bis(5'-adenosyl)-triphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 149	148	Bis(5'-adenosyl)-triphosphatase		PRO_0000288472
Regions
	Domain	2 – 109	108	HIT

Sequences

	Sequence		Length	Mass (Da)
	Q1KZG4-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: C7E39594A5982F24	FASTA	149	16,951
	10 20 30 40 50 60 MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFRDM SPEEVADLFQ 70 80 90 100 110 120 AAQRVGTVVE KHFQGTSLTF SMQDGPEAGQ TVKHVHVHIL PRKAGDFHRN DSIYDALEKH 130 140 DREDKDSPAL WRSEEEMAAE AAALRVYFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance." Sugimoto M., Fujikawa A., Womack J.E., Sugimoto Y. Proc. Natl. Acad. Sci. U.S.A. 103:6454-6459(2006) [PubMed: 16611727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Comparative genomic mapping of the bovine Fragile Histidine Triad (FHIT) tumour suppressor gene: characterization of a 2 Mb BAC contig covering the locus, complete annotation of the gene, analysis of cDNA and of physiological expression profiles." Uboldi C., Guidi E., Roperto S., Russo V., Roperto F., Di Meo G.P., Iannuzzi L., Floriot S., Boussaha M., Eggen A., Ferretti L. BMC Genomics 7:123-123(2006) [PubMed: 16719907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-116, TISSUE SPECIFICITY.
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ335870 mRNA. Translation: ABC61315.1. DQ310184 mRNA. Translation: ABC61471.1. DQ310183 mRNA. Translation: ABC61470.1. DQ310181 mRNA. Translation: ABC61468.1. DQ310182 mRNA. Translation: ABC61469.1. DQ310179 , DQ310176, DQ310177, DQ310178 Genomic DNA. Translation: ABC68307.1. BC147993 mRNA. Translation: AAI47994.1.
IPI	IPI00696363.
RefSeq	NP_001035736.1.
UniGene	Bt.26240
3D structure databases
SMR	Q1KZG4. Positions 2-131.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000014418. Bos taurus. [Contig view]
GeneID	692183.
KEGG	bta:692183.
Phylogenomic databases
HOVERGEN	Q1KZG4.
OMA	Q1KZG4. HILPRKA.
Enzyme and pathway databases
BRENDA	3.6.1.29. 251.
Family and domain databases
InterPro	IPR011151. His_triad_motif. IPR019808. Histidine_triad_CS. IPR001310. Histidine_triad_HIT. [Graphical view]
Gene3D	G3DSA:3.30.428.10. His_triad_motif. 1 hit.
PANTHER	PTHR23089. HIT. 1 hit.
Pfam	PF01230. HIT. 1 hit. [Graphical view]
PROSITE	PS00892. HIT_1. 1 hit. PS51084. HIT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FHIT_BOVIN

Accession

Primary (citable) accession number: Q1KZG4
Secondary accession number(s): A6QLK1, Q1KZS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)