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Protein

Sulfite reductase [NADPH] subunit beta

Gene

sir1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of sulfite to sulfide, one of several activities required for the biosynthesis of L-cysteine from sulfate.By similarity

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.By similarity

Cofactori

Protein has several cofactor binding sites:
  • sirohemeBy similarityNote: Binds 1 siroheme per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] subunit beta (sir1), Probable sulfite reductase [NADPH] flavoprotein component (SPCC584.01c)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1328Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi1334Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi1373Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi1377Iron (siroheme axial ligand)By similarity1
Metal bindingi1377Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • cysteine biosynthetic process Source: UniProtKB-KW
  • hydrogen sulfide biosynthetic process Source: PomBase
  • methionine biosynthetic process Source: UniProtKB-KW
  • sulfate assimilation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] subunit beta (EC:1.8.1.2)
Gene namesi
Name:sir1
ORF Names:SPAC10F6.01c, SPAC4C5.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC10F6.01c.
PomBaseiSPAC10F6.01c. sir1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • sulfite reductase complex (NADPH) Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003168601 – 1473Sulfite reductase [NADPH] subunit betaAdd BLAST1473

Proteomic databases

MaxQBiQ1K9C2.
PRIDEiQ1K9C2.

PTM databases

SwissPalmiQ1K9C2.

Interactioni

Subunit structurei

Alpha(2)-beta2. The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).By similarity

Protein-protein interaction databases

BioGridi279361. 4 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ1K9C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini728 – 876Flavodoxin-likePROSITE-ProRule annotationAdd BLAST149

Sequence similaritiesi

Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000167149.
InParanoidiQ1K9C2.
KOiK00381.
OMAiHEIWLKD.
OrthoDBiEOG092C1RRH.
PhylomeDBiQ1K9C2.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
3.90.480.10. 2 hits.
InterProiIPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like_dom.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
PF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00397. SIROHAEM.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
SSF55124. SSF55124. 2 hits.
PROSITEiPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1K9C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKASINNS QEAVSRIAFR CSDALYVVHP NNSILNGALT ESLKDLKKFE
60 70 80 90 100
TLNVSGKVPH VLPLKSHADP FAHIADAILA EEEVASTQPK QITSVVASAD
110 120 130 140 150
ALFFATPHLY KLAHEPLVAH VAIESTEAFD FASVRDTGFV ILFSGNRPGD
160 170 180 190 200
SSEAALEDTL ETASLAHRLA LKLNTGVLHF YSPVYDTTAA LENIETLPSK
210 220 230 240 250
EDAQHARVAH IPIEEKQEDS EKEGNIKEAF VPPKFDQPER DAATSEYLES
260 270 280 290 300
LSIKPFEYSG SDDATDVLLV FGSAASELAK AAVTSSVAVA IVRVLRPWLP
310 320 330 340 350
SKLQEVLPTS TKRLTVLEPI TSLPRKWDPL YLDVLSSFVA SGSSIELFAV
360 370 380 390 400
RYGLSSSEQA TEIIKAVRDN LSGALKPSLV CDFTDGVSQV FVPTPPSIEE
410 420 430 440 450
AYHKLLHRVF KSRLNIVNDP ASSATKQNIP SRLIISPQFA LGSVLEYENQ
460 470 480 490 500
RRAFCDEVAT LLKEKNSSVS SESLEVLSNW IVSVDNLESP VDPELVISEL
510 520 530 540 550
KKDSSAPIKS LLDRSEFFTN VSHWIIGSDA WAYDLGNSAL HQVLCLEKNV
560 570 580 590 600
NLLIVDTQPY STREAVRSSS RKKDIGLYAM NFGNAYVAST ALYSSYTQLI
610 620 630 640 650
SALLEADKFK GPSVVLAYLP YHSADDDAIT VLQETKKAVD IGYWPLYRWT
660 670 680 690 700
PALEDGEYSD FKLDSERIRR ELKTFLERDN YLTQLTLRVP SLARTLTQSF
710 720 730 740 750
GAEVRHQQNV DSRNALNKLI EGLSGPPLTI LFASDGGTAE NVAKRLQNRA
760 770 780 790 800
SARGSKCKIM AMDDFPIEEL GNEKNVVVLV STAGQGEFPQ NGREFWEAIK
810 820 830 840 850
GADLNLSELK YGVFGFGDYE YWPRKEDKIY YNRPGKQLDA RFVELGAAPL
860 870 880 890 900
VTLGLGNDQD PDGWETAYNL WEPELWKALG LDNVEIDIDE PKPITNEDIK
910 920 930 940 950
QASNFLRGTI FEGLADESTG ALAESDCQLT KFHGIYMQDD RDIRDERKKQ
960 970 980 990 1000
GLEPAYGFMI RARMPAGVCT PEQWIAMDDI STKWGNHTLK ITTRQTFQWH
1010 1020 1030 1040 1050
GVLKKNLRNT IRNIVKVFLT TLGACGDVAR NVTCSSTPNN ETIHDQLFAV
1060 1070 1080 1090 1100
SKQISNELLP TTSSYHEIWI EDPETVEKRK VAGEAVQDVE PLYGPTYLPR
1110 1120 1130 1140 1150
KFKVGVAAPP YNDVDVYTND VALIAIIEND KVLGFNVGIG GGMGTTHNNK
1160 1170 1180 1190 1200
KTYPRLATVV GYVLTDKIME VVKAILIVQR DNGDRENRKH ARLKYTVDTL
1210 1220 1230 1240 1250
GVSTFVEKVE EVLGYKFEEA RDHPQFIKNH DDFEGWHKTE KNKYWRSIFV
1260 1270 1280 1290 1300
ENGRIENNGI LQFKTGLREL AERLYTEKSE AEFRLTANQH VILFNVAENE
1310 1320 1330 1340 1350
LGWINEHMAK YKLDNNAFSG LRLSSAACVA LPTCGLAMAE SERYLPKLIT
1360 1370 1380 1390 1400
KVEEIVYEAG LQKDSIVMRM TGCPNGCSRP WVAEIACVGK APNTYNLMLG
1410 1420 1430 1440 1450
GGFYGQRLNK LYRSSVQEKE ILNLLRPLIK RYALEREDGE HFGDWVIRAG
1460 1470
IITAVENGGA NGAVHEGVSP EAF
Length:1,473
Mass (Da):163,844
Last modified:May 30, 2006 - v1
Checksum:iA9A310E7310DF1F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11176.1.
RefSeqiNP_593252.1. NM_001018649.2.

Genome annotation databases

EnsemblFungiiSPAC10F6.01c.1; SPAC10F6.01c.1:pep; SPAC10F6.01c.
GeneIDi2542920.
KEGGispo:SPAC10F6.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11176.1.
RefSeqiNP_593252.1. NM_001018649.2.

3D structure databases

ProteinModelPortaliQ1K9C2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279361. 4 interactors.

PTM databases

SwissPalmiQ1K9C2.

Proteomic databases

MaxQBiQ1K9C2.
PRIDEiQ1K9C2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC10F6.01c.1; SPAC10F6.01c.1:pep; SPAC10F6.01c.
GeneIDi2542920.
KEGGispo:SPAC10F6.01c.

Organism-specific databases

EuPathDBiFungiDB:SPAC10F6.01c.
PomBaseiSPAC10F6.01c. sir1.

Phylogenomic databases

HOGENOMiHOG000167149.
InParanoidiQ1K9C2.
KOiK00381.
OMAiHEIWLKD.
OrthoDBiEOG092C1RRH.
PhylomeDBiQ1K9C2.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.

Miscellaneous databases

PROiQ1K9C2.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
3.90.480.10. 2 hits.
InterProiIPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like_dom.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
PF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00397. SIROHAEM.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
SSF55124. SSF55124. 2 hits.
PROSITEiPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMET5_SCHPO
AccessioniPrimary (citable) accession number: Q1K9C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 30, 2006
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.