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Protein

Neutral cholesterol ester hydrolase 1

Gene

NCEH1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei191 – 1911PROSITE-ProRule annotation
Active sitei348 – 3481PROSITE-ProRule annotation
Active sitei378 – 3781PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-BTA-73923. Lipid digestion, mobilization, and transport.

Protein family/group databases

ESTHERibovin-nceh1. Hormone-sensitive_lipase_like_1.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral cholesterol ester hydrolase 1 (EC:3.1.1.-)
Short name:
NCEH
Alternative name(s):
Arylacetamide deacetylase-like 1
Gene namesi
Name:NCEH1
Synonyms:AADACL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence analysis
Transmembranei5 – 2521Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini26 – 408383LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Neutral cholesterol ester hydrolase 1PRO_0000352850Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence analysis
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ1JQE6.
PRIDEiQ1JQE6.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026745.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi113 – 1153Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity

Sequence similaritiesi

Belongs to the 'GDXG' lipolytic enzyme family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1515. Eukaryota.
COG0657. LUCA.
GeneTreeiENSGT00550000074556.
HOGENOMiHOG000033738.
HOVERGENiHBG058974.
InParanoidiQ1JQE6.
KOiK14349.
OMAiALGQQFT.
OrthoDBiEOG7HB599.
TreeFamiTF314978.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR017157. Arylacetamide_deacetylase.
IPR033140. Lipase_GDXG_put_SER_AS.
[Graphical view]
PfamiPF07859. Abhydrolase_3. 2 hits.
[Graphical view]
PIRSFiPIRSF037251. Arylacetamide_deacetylase. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1JQE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSSCVLLTA LLALAAYYIY IPLPSSVSDP WKLMLLDATF RSAQQVSNLI
60 70 80 90 100
HFLGLSHHLL ALNFIIVSFG KKSAWSSAQV KVTDTDFDGV EVRVFEGPPK
110 120 130 140 150
PEEPLKRSIV YIHGGGWALA SAKIRYYDEL CTTMAEELNA VIVSIEYRLV
160 170 180 190 200
PKVYFPEQIH DVVHATKYFL QPEVLHKYSV DPGRVGISGD SAGGNLAAAL
210 220 230 240 250
GQQFNQDTNL KNKLKVQALI YPVLQALDFN TPSYQQNMNT PILPRYVMVK
260 270 280 290 300
YWVDYFNGNY DFVQAMIVNN HTSLDVDEAS ALRARLNWTS LLPTSITKNY
310 320 330 340 350
KPVMQTTGNS RIVQEIPQLL DARSAPLIAD QEVLQHLPKT YILTCEHDVL
360 370 380 390 400
RDDGIMYAKR LESAGVEVTL DHFEDGFHGC MIFTSWPTNF SVGIRTRNSY

IKWLDQNL
Length:408
Mass (Da):46,026
Last modified:April 17, 2007 - v2
Checksum:i91F33861B07ED838
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC115993 mRNA. Translation: AAI15994.2.
RefSeqiNP_001116506.1. NM_001123034.1.
UniGeneiBt.64890.

Genome annotation databases

EnsembliENSBTAT00000026745; ENSBTAP00000026745; ENSBTAG00000020073.
GeneIDi534212.
KEGGibta:534212.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC115993 mRNA. Translation: AAI15994.2.
RefSeqiNP_001116506.1. NM_001123034.1.
UniGeneiBt.64890.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026745.

Protein family/group databases

ESTHERibovin-nceh1. Hormone-sensitive_lipase_like_1.

Proteomic databases

PaxDbiQ1JQE6.
PRIDEiQ1JQE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026745; ENSBTAP00000026745; ENSBTAG00000020073.
GeneIDi534212.
KEGGibta:534212.

Organism-specific databases

CTDi57552.

Phylogenomic databases

eggNOGiKOG1515. Eukaryota.
COG0657. LUCA.
GeneTreeiENSGT00550000074556.
HOGENOMiHOG000033738.
HOVERGENiHBG058974.
InParanoidiQ1JQE6.
KOiK14349.
OMAiALGQQFT.
OrthoDBiEOG7HB599.
TreeFamiTF314978.

Enzyme and pathway databases

ReactomeiR-BTA-73923. Lipid digestion, mobilization, and transport.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR017157. Arylacetamide_deacetylase.
IPR033140. Lipase_GDXG_put_SER_AS.
[Graphical view]
PfamiPF07859. Abhydrolase_3. 2 hits.
[Graphical view]
PIRSFiPIRSF037251. Arylacetamide_deacetylase. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.

Entry informationi

Entry nameiNCEH1_BOVIN
AccessioniPrimary (citable) accession number: Q1JQE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: April 17, 2007
Last modified: July 6, 2016
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.