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Protein

Mitotic checkpoint protein BUB3

Gene

BUB3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Enzyme and pathway databases

ReactomeiR-BTA-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-BTA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-BTA-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-BTA-179409. APC-Cdc20 mediated degradation of Nek2A.
R-BTA-2467813. Separation of Sister Chromatids.
R-BTA-2500257. Resolution of Sister Chromatid Cohesion.
R-BTA-5663220. RHO GTPases Activate Formins.
R-BTA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic checkpoint protein BUB3
Gene namesi
Name:BUB3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

  • Nucleus By similarity
  • Chromosomecentromerekinetochore By similarity

  • Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002540251 – 326Mitotic checkpoint protein BUB3Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei179N6-acetyllysineBy similarity1
Modified residuei211PhosphoserineBy similarity1
Cross-linki216Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.By similarity

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ1JQB2.
PeptideAtlasiQ1JQB2.
PRIDEiQ1JQB2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017913.

Interactioni

Subunit structurei

Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000051164.

Structurei

3D structure databases

ProteinModelPortaliQ1JQB2.
SMRiQ1JQB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati5 – 43WD 1Add BLAST39
Repeati46 – 83WD 2Add BLAST38
Repeati86 – 124WD 3Add BLAST39
Repeati128 – 163WD 4Add BLAST36
Repeati223 – 262WD 5Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat BUB3 family.Curated
Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1036. Eukaryota.
ENOG410XRBG. LUCA.
GeneTreeiENSGT00530000063440.
HOGENOMiHOG000208822.
HOVERGENiHBG002942.
InParanoidiQ1JQB2.
KOiK02180.
OMAiEGEKSHE.
OrthoDBiEOG091G0AB8.
TreeFamiTF105454.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1JQB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGSNEFKLN QPPEDGISSV KFSPNTSQFL LVSSWDTSVR LYDVPANSMR
60 70 80 90 100
LKYQHTGAVL DCAFYDPTHA WSGGLDHQLK MHDLNTDQEN LVGTHDAPIR
110 120 130 140 150
CVEYCPEVNV MVTGSWDQTV KLWDPRTPCN AGTFSQPEKV YTLSVSGDRL
160 170 180 190 200
IVGTAGRRVL VWDLRNMGYV QQRRESSLKY QTRCIRAFPN KQGYVLSSIE
210 220 230 240 250
GRVAVEYLDP SPEVQKKKYA FKCHRLKENN IEQIYPVNAI SFHNIHNTFA
260 270 280 290 300
TGGSDGFVNI WDPFNKKRLC QFHRYPTSIA SLAFSNDGTT LAIASSYMYE
310 320
MDDTEHPEDG IFIRQVTDAE TKPKST
Length:326
Mass (Da):36,955
Last modified:June 13, 2006 - v1
Checksum:iF1EA57368E5A6345
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030579 mRNA. Translation: ABQ13019.1.
BC116090 mRNA. Translation: AAI16091.1.
RefSeqiNP_001069645.1. NM_001076177.1.
UniGeneiBt.26981.

Genome annotation databases

EnsembliENSBTAT00000057027; ENSBTAP00000051164; ENSBTAG00000017913.
GeneIDi539594.
KEGGibta:539594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030579 mRNA. Translation: ABQ13019.1.
BC116090 mRNA. Translation: AAI16091.1.
RefSeqiNP_001069645.1. NM_001076177.1.
UniGeneiBt.26981.

3D structure databases

ProteinModelPortaliQ1JQB2.
SMRiQ1JQB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000051164.

Proteomic databases

PaxDbiQ1JQB2.
PeptideAtlasiQ1JQB2.
PRIDEiQ1JQB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000057027; ENSBTAP00000051164; ENSBTAG00000017913.
GeneIDi539594.
KEGGibta:539594.

Organism-specific databases

CTDi9184.

Phylogenomic databases

eggNOGiKOG1036. Eukaryota.
ENOG410XRBG. LUCA.
GeneTreeiENSGT00530000063440.
HOGENOMiHOG000208822.
HOVERGENiHBG002942.
InParanoidiQ1JQB2.
KOiK02180.
OMAiEGEKSHE.
OrthoDBiEOG091G0AB8.
TreeFamiTF105454.

Enzyme and pathway databases

ReactomeiR-BTA-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-BTA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-BTA-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-BTA-179409. APC-Cdc20 mediated degradation of Nek2A.
R-BTA-2467813. Separation of Sister Chromatids.
R-BTA-2500257. Resolution of Sister Chromatid Cohesion.
R-BTA-5663220. RHO GTPases Activate Formins.
R-BTA-68877. Mitotic Prometaphase.

Gene expression databases

BgeeiENSBTAG00000017913.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBUB3_BOVIN
AccessioniPrimary (citable) accession number: Q1JQB2
Secondary accession number(s): A5D9G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 13, 2006
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.