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Protein

Pectinesterase/pectinesterase inhibitor 18

Gene

PME18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin. Inhibits the elongation phase of protein synthesis.1 Publication

Catalytic activityi

Pectin + n H2O = n methanol + pectate.
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At5g52320), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Pectinesterase (AXX17_At2g33410), Probable pectinesterase 55 (PME55), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase (AXX17_At4g18720), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At5g47900), Pectinesterase (At3g14310), Pectinesterase (AXX17_At4g38050), Pectinesterase (AXX17_At5g07100), Pectinesterase/pectinesterase inhibitor 18 (PME18), Pectinesterase (AXX17_At2g14510), Pectinesterase (AXX17_At3g43380), Pectinesterase (AXX17_At5g64270), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Pectinesterase (F14I3.7), Probable pectinesterase 29 (PME29), Pectinesterase, Pectinesterase (AXX17_At4g38040), Probable pectinesterase 8 (PME8), Pectinesterase (AXX17_At5g27870), Pectinesterase (AXX17_At5g18820), Pectinesterase (AXX17_At2g45340), Pectinesterase 31 (PME31), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At1g11650), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At5g04430), Pectinesterase (At3g62170), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Pectinesterase (T27B3.30), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Pectinesterase QRT1 (QRT1), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At5g61190), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At3g06710), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Pectinesterase (AXX17_At1g44540), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g40510), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Pectinesterase (AXX17_At3g26070), Probable pectinesterase 68 (PME68), Pectinesterase, Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At5g54740), Probable pectinesterase 67 (PME67), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase 56 (PME56), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase 53 (PME53), Pectinesterase (AXX17_At5g07090), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase (AXX17_At2g22270), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g45990), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Pectinesterase (AXX17_At1g64100), Probable pectinesterase 50 (PME50), Pectinesterase (AXX17_At3g17940), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Pectinesterase (AXX17_At2g45050), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At3g30550), PME26 (AXX17_At3g14890), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At2g17140), Pectinesterase (AXX17_At5g19650), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At4g02900), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Pectinesterase (AXX17_At3g05180), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Pectinesterase (AXX17_At2g22280), Pectinesterase (AXX17_At1g51200), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Pectinesterase (AXX17_At4g02930), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase, Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At1g11900), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At3g10570), Putative pectinesterase 11 (PME11), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At5g07110), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At5g50310), Pectinesterase, Pectinesterase (AXX17_At1g51190), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At3g05190), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g09310), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At1g04730), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Pectinesterase (AXX17_At1g11660), Pectinesterase (AXX17_At5g26780)
  2. Pectate lyase (AXX17_At3g26720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Pectate lyase (AXX17_At3g09180), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At4g15660), Probable pectate lyase 22 (At5g63180), Pectate lyase (T5E8_80), Pectate lyase (AXX17_At3g00310), Pectate lyase (At3g07010), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Pectate lyase (AXX17_At2g01680), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Pectate lyase (At5g04310), Pectate lyase (AXX17_At5g03700), Pectate lyase (At4g13710), Pectate lyase (AXX17_At3g49720), Pectate lyase (At3g01270), Pectate lyase (At5g04310), Pectate lyase (AXX17_At1g12300), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Pectate lyase (AXX17_At5g08800), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At5g62760), Pectate lyase (AXX17_At3g47590), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At1g61710), Probable pectate lyase 20 (At5g48900), Pectate lyase (AXX17_At5g47570), Probable pectate lyase 6 (At2g02720), Pectate lyase (AXX17_At3g06910), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase (At2g02720), Pectate lyase (AXX17_At1g04020), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase, Pectate lyase (AXX17_At1g30780), Probable pectate lyase 19 (At5g15110), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Pectate lyase (AXX17_At3g26200), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 12 (At3g53190), Pectate lyase (At4g24780), Pectate lyase (AXX17_At4g25610), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Putative pectate lyase 11 (At3g27400), Pectate lyase (AXX17_At1g15010), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g14600), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei321Substrate; for pectinesterase activityBy similarity1
Binding sitei351Substrate; for pectinesterase activityBy similarity1
Sitei373Transition state stabilizerBy similarity1
Active sitei374Proton donor; for pectinesterase activityPROSITE-ProRule annotation1
Active sitei395Nucleophile; for pectinesterase activityPROSITE-ProRule annotation1
Binding sitei463Substrate; for pectinesterase activityBy similarity1
Binding sitei465Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Aspartyl esterase, Fungicide, Hydrolase, Protein synthesis inhibitor, Toxin

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT1G11580-MONOMER.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase/pectinesterase inhibitor 18
Alternative name(s):
AtPMEpcrA
Cleaved into the following 2 chains:
Alternative name(s):
Pectin methylesterase inhibitor 18
Alternative name(s):
Pectin methylesterase 18
Pectin methylesterase 4
Short name:
AtPME4
Ribosome-inactivating protein
Gene namesi
Name:PME18
Synonyms:ARATH4
Ordered Locus Names:At1g11580
ORF Names:T23J18.23, T23J18.24, T23J18.33
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G11580.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • cytosol Source: TAIR
  • extracellular region Source: UniProtKB-KW
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Sequence analysisAdd BLAST34
ChainiPRO_000037018235 – 557Pectinesterase/pectinesterase inhibitor 18Add BLAST523
ChainiPRO_000037018335 – 242Pectinesterase inhibitor 18Sequence analysisAdd BLAST208
ChainiPRO_0000370184243 – 557Bifunctional pectinesterase 18/rRNA N-glycosylaseAdd BLAST315

Proteomic databases

PaxDbiQ1JPL7.
PRIDEiQ1JPL7.

PTM databases

iPTMnetiQ1JPL7.
SwissPalmiQ1JPL7.

Expressioni

Tissue specificityi

Expressed in siliques, flowers, floral stems, rosette leaves and roots.2 Publications

Developmental stagei

Expressed during early seed development and late developmental phases of siliques.1 Publication

Gene expression databases

GenevisibleiQ1JPL7. AT.

Interactioni

Protein-protein interaction databases

BioGridi22941. 1 interactor.
STRINGi3702.AT1G11580.1.

Structurei

3D structure databases

ProteinModelPortaliQ1JPL7.
SMRiQ1JPL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 203Pectinesterase inhibitor 18Add BLAST157
Regioni246 – 543Pectinesterase 18Add BLAST298

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi292 – 295Poly-Lys4

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II3N. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ1JPL7.
KOiK01051.
OMAiNHIDPAG.
OrthoDBiEOG093608YE.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1JPL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNSNQPLLS KPKSLKHKNL CLVLSFVAIL GSVAFFTAQL ISVNTNNNDD
60 70 80 90 100
SLLTTSQICH GAHDQDSCQA LLSEFTTLSL SKLNRLDLLH VFLKNSVWRL
110 120 130 140 150
ESTMTMVSEA RIRSNGVRDK AGFADCEEMM DVSKDRMMSS MEELRGGNYN
160 170 180 190 200
LESYSNVHTW LSSVLTNYMT CLESISDVSV NSKQIVKPQL EDLVSRARVA
210 220 230 240 250
LAIFVSVLPA RDDLKMIISN RFPSWLTALD RKLLESSPKT LKVTANVVVA
260 270 280 290 300
KDGTGKFKTV NEAVAAAPEN SNTRYVIYVK KGVYKETIDI GKKKKNLMLV
310 320 330 340 350
GDGKDATIIT GSLNVIDGST TFRSATVAAN GDGFMAQDIW FQNTAGPAKH
360 370 380 390 400
QAVALRVSAD QTVINRCRID AYQDTLYTHT LRQFYRDSYI TGTVDFIFGN
410 420 430 440 450
SAVVFQNCDI VARNPGAGQK NMLTAQGRED QNQNTAISIQ KCKITASSDL
460 470 480 490 500
APVKGSVKTF LGRPWKLYSR TVIMQSFIDN HIDPAGWFPW DGEFALSTLY
510 520 530 540 550
YGEYANTGPG ADTSKRVNWK GFKVIKDSKE AEQFTVAKLI QGGLWLKPTG

VTFQEWL
Length:557
Mass (Da):61,687
Last modified:May 3, 2011 - v3
Checksum:iB1A3C1620DCC127B
GO

Sequence cautioni

The sequence AAF16636 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF16637 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14S → Y in AAK59760 (PubMed:14593172).Curated1
Sequence conflicti460F → Y in AAC02973 (PubMed:9767082).Curated1
Sequence conflicti467L → E in AAC02973 (PubMed:9767082).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011661 Genomic DNA. Translation: AAF16636.1. Sequence problems.
AC011661 Genomic DNA. Translation: AAF16637.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28755.1.
AY037175 mRNA. Translation: AAK59760.1.
AY048217 mRNA. Translation: AAK82480.1.
BT025336 mRNA. Translation: ABF57292.1.
AF033205 Genomic DNA. Translation: AAC02973.1.
PIRiA86249.
RefSeqiNP_172624.1. NM_101031.3.
UniGeneiAt.24510.

Genome annotation databases

EnsemblPlantsiAT1G11580.1; AT1G11580.1; AT1G11580.
GeneIDi837701.
GrameneiAT1G11580.1; AT1G11580.1; AT1G11580.
KEGGiath:AT1G11580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011661 Genomic DNA. Translation: AAF16636.1. Sequence problems.
AC011661 Genomic DNA. Translation: AAF16637.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28755.1.
AY037175 mRNA. Translation: AAK59760.1.
AY048217 mRNA. Translation: AAK82480.1.
BT025336 mRNA. Translation: ABF57292.1.
AF033205 Genomic DNA. Translation: AAC02973.1.
PIRiA86249.
RefSeqiNP_172624.1. NM_101031.3.
UniGeneiAt.24510.

3D structure databases

ProteinModelPortaliQ1JPL7.
SMRiQ1JPL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22941. 1 interactor.
STRINGi3702.AT1G11580.1.

PTM databases

iPTMnetiQ1JPL7.
SwissPalmiQ1JPL7.

Proteomic databases

PaxDbiQ1JPL7.
PRIDEiQ1JPL7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G11580.1; AT1G11580.1; AT1G11580.
GeneIDi837701.
GrameneiAT1G11580.1; AT1G11580.1; AT1G11580.
KEGGiath:AT1G11580.

Organism-specific databases

TAIRiAT1G11580.

Phylogenomic databases

eggNOGiENOG410II3N. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ1JPL7.
KOiK01051.
OMAiNHIDPAG.
OrthoDBiEOG093608YE.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BioCyciARA:AT1G11580-MONOMER.

Miscellaneous databases

PROiQ1JPL7.

Gene expression databases

GenevisibleiQ1JPL7. AT.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME18_ARATH
AccessioniPrimary (citable) accession number: Q1JPL7
Secondary accession number(s): O49007
, Q94AF4, Q94C39, Q9LPX8, Q9LPX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 3, 2011
Last modified: November 30, 2016
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.