Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial

Gene

NDB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alternative NADH-ubiquinone oxidoreductase which catalyzes the oxidation of mitochondrial NADH does not translocate protons across the inner mitochondrial membrane (By similarity). Calcium-dependent NAD(P)H dehydrogenase. Binds calcium ions.By similarity1 Publication

Catalytic activityi

NADH + ubiquinone = NAD+ + ubiquinol.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Enzyme regulationi

Activity is calcium-dependent with a more pronounced effect at higher pH.1 Publication

pH dependencei

Optimum pH is 6.8-7.2 with NADPH as substrate and 6.8 with NADH as substrate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi51 – 81FADBy similarityAdd BLAST31
Nucleotide bindingi215 – 251NADBy similarityAdd BLAST37
Calcium bindingi385 – 396Add BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandCalcium, FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciARA:AT4G28220-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial (EC:1.6.5.9)
Alternative name(s):
External alternative NADH dehydrogenase NDB1
NADH:ubiquinone reductase (non-electrogenic) NDB1
Gene namesi
Name:NDB1
Ordered Locus Names:At4g28220
ORF Names:F26K10.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G28220.
TAIRilocus:2123713. AT4G28220.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi387D → A: Impaired calcium binding and loss of NADH oxidase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionSequence analysisAdd BLAST35
ChainiPRO_000041950536 – 571External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrialAdd BLAST536

Proteomic databases

PaxDbiQ1JPL4.

PTM databases

SwissPalmiQ1JPL4.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, cotyledons, leaves, stems, buds and flowers.2 Publications

Gene expression databases

ExpressionAtlasiQ1JPL4. baseline and differential.
GenevisibleiQ1JPL4. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G28220.1.

Structurei

3D structure databases

ProteinModelPortaliQ1JPL4.
SMRiQ1JPL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini372 – 407EF-handPROSITE-ProRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi562 – 571Microbody targeting signal10

Sequence similaritiesi

Belongs to the NADH dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2495. Eukaryota.
COG1252. LUCA.
HOGENOMiHOG000182501.
InParanoidiQ1JPL4.
KOiK17871.
OMAiYAWRSVY.
OrthoDBiEOG0936051A.
PhylomeDBiQ1JPL4.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR036188. FAD/NAD-bd_sf.
IPR023753. FAD/NAD-binding_dom.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
PfamiView protein in Pfam
PF07992. Pyr_redox_2. 1 hit.
PRINTSiPR00469. PNDRDTASEII.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1JPL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLSSLGRA SRSAPLASKL LLLGTLSGGS IVAYADANEE ANKKEEHKKK
60 70 80 90 100
KVVVLGTGWA GISFLKDLDI TSYDVQVVSP QNYFAFTPLL PSVTCGTVEA
110 120 130 140 150
RSIVESVRNI TKKKNGEIEL WEADCFKIDH VNQKVHCRPV FKDDPEASQE
160 170 180 190 200
FSLGYDYLIV AVGAQVNTFG TPGVLENCHF LKEVEDAQRI RRGVIDCFEK
210 220 230 240 250
AILPGLTEEQ RRRKLHFVIV GGGPTGVEFA AELHDFIIED ITKIYPSVKE
260 270 280 290 300
LVKITLIQSG DHILNTFDER ISSFAEQKFT RDGIDVQTGM RVMSVTDKDI
310 320 330 340 350
TVKVKSSGEL VSIPHGLILW STGVGTRPVI SDFMEQVGQG GRRAVATNEW
360 370 380 390 400
LQVTGCENVY AVGDCASIAQ RKILGDIANI FKAADADNSG TLTMEELEGV
410 420 430 440 450
VDDIIVRYPQ VELYLKSKHM RHINDLLADS EGNARKEVDI EAFKLALSEA
460 470 480 490 500
DSQMKTLPAT AQVAAQQGAY LAKCFNRMEQ CKELPEGPKR FRTGGHHQFR
510 520 530 540 550
PFQYKHFGQF APLGGDQAAA ELPGDWVSAG KSAQWLWYSV YASKQVSWRT
560 570
RALVVSDWTR RYIFGRDSSR I
Length:571
Mass (Da):63,314
Last modified:June 13, 2006 - v1
Checksum:i5B8B7925B95A2D91
GO

Sequence cautioni

The sequence CAB79624 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39E → A in AAM63256 (Ref. 4) Curated1
Sequence conflicti175L → I in AAM63256 (Ref. 4) Curated1
Sequence conflicti386A → V in AAM63256 (Ref. 4) Curated1
Sequence conflicti540V → I in AAM63256 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161572 Genomic DNA. Translation: CAB79624.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85455.1.
BT025339 mRNA. Translation: ABF57295.1.
AY086046 mRNA. Translation: AAM63256.1.
PIRiT09038.
RefSeqiNP_567801.1. NM_118962.5.
UniGeneiAt.69904.
At.71073.

Genome annotation databases

EnsemblPlantsiAT4G28220.1; AT4G28220.1; AT4G28220.
GeneIDi828937.
GrameneiAT4G28220.1; AT4G28220.1; AT4G28220.
KEGGiath:AT4G28220.

Similar proteinsi

Entry informationi

Entry nameiNDB1_ARATH
AccessioniPrimary (citable) accession number: Q1JPL4
Secondary accession number(s): Q8LDE7, Q9M0I5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: June 13, 2006
Last modified: October 25, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families