Q1JPJ8 (THOP1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thimet oligopeptidase
EC=3.4.24.15

Gene names

Name:

THOP1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	687 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein and generate amyloidogenic fragments By similarity.
Catalytic activity	Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 687	686	Thimet oligopeptidase		PRO_0000263069
Sites
	Active site	474	1	By similarity
	Metal binding	473	1	Zinc; catalytic By similarity
	Metal binding	477	1	Zinc; catalytic By similarity
	Metal binding	480	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	278	1	Phosphotyrosine By similarity
	Modified residue	538	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	50	1	C → R in AAI49984. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q1JPJ8 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 70627C332F5A7B8B	FASTA	687	78,139
	10 20 30 40 50 60 MKPPAACTGD ALDVVAPCSA VNHLRWDLSA QQIAELTTEL IEQTKRVYDC VGAQEPQDVS 70 80 90 100 110 120 YENTLKALAD VEVSYTVQRN ILDFPQHVSP SKDIRTASTE ADKKLSEFDV EMSMRQDVYQ 130 140 150 160 170 180 RIVWLQEKVQ KDSLRPEASR YLERLIKLGR RNGLHLPEET QEKIKSIKKK LSLLCIDFNK 190 200 210 220 230 240 NLNEDTTFLP FTREELGGLP EDFLNSLEKT EDEKLKVTLK YPHYFPLLKK CHVPETRRKV 250 260 270 280 290 300 EEAFNCRCKE ENCAILRELV RLRAQKSRLL GFSTHADYVL EMNMAKTSQV VATFLDELAQ 310 320 330 340 350 360 KLKPLGEQER AVILELKRAE CEQRGLAFDG RINAWDMRYY MNQVEETRYR VDQNLLKEYF 370 380 390 400 410 420 PMQVVTRGLL GIYQELLGLS FQLEEGAAVW HEDVALYAVR DAASGKLIGK FYLDLYPREG 430 440 450 460 470 480 KYGHAACFGL QPGCLRKDGS RQIAIAAMVA NFTKPTPDAP SLLQHDEVET YFHEFGHVMH 490 500 510 520 530 540 QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEAEPLLR MSQHYRTGSS IPQELLDKLI 550 560 570 580 590 600 KSRQANTGLF NLRQIVLAKV DQALHTQTAA DPAKEYARLC QEILGVPATP GTNMPATFGH 610 620 630 640 650 660 LAGGYDAQYY GYLWSEVYSA DMFHTRFKQE GVLSGKVGMD YRSCILRPGG SEDASVMLKL 670 680 FLGRDPKQDA FLLSKGLQVE GCEPPAC

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thymus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT025355 mRNA. Translation: ABF57311.1. BC149983 mRNA. Translation: AAI49984.1.
IPI	IPI00867151.
RefSeq	NP_001029163.2. NM_001033991.2.
UniGene	Bt.21827.
3D structure databases
ProteinModelPortal	Q1JPJ8.
SMR	Q1JPJ8. Positions 24-677.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1JPJ8.
Proteomic databases
PRIDE	Q1JPJ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000027246; ENSBTAP00000027246; ENSBTAG00000020446.
GeneID	510889.
KEGG	bta:510889.
Organism-specific databases
CTD	7064.
Phylogenomic databases
eggNOG	maNOG11722.
GeneTree	ENSGT00550000074738.
HOVERGEN	HBG000238.
InParanoid	Q1JPJ8.
OrthoDB	EOG4CG07P.
PhylomeDB	Q1JPJ8.
Family and domain databases
InterPro	IPR024079. MetalloPept_cat_dom. IPR024077. Neurolysin/TOP_dom2. IPR024080. Neurolysin/TOP_N. IPR001567. Pept_M3A_M3B. [Graphical view]
Gene3D	G3DSA:1.10.1370.10. G3DSA:1.10.1370.10. 2 hits. G3DSA:1.20.1050.40. G3DSA:1.20.1050.40. 1 hit. G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
KO	K01392.
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THOP1_BOVIN

Accession

Primary (citable) accession number: Q1JPJ8
Secondary accession number(s): A6QQT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 45 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program