Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q1JPJ8 (THOP1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thimet oligopeptidase

EC=3.4.24.15
Gene names
Name:THOP1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length687 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein and generate amyloidogenic fragments By similarity.

Catalytic activity

Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the peptidase M3 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpeptide metabolic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 687687Thimet oligopeptidase
PRO_0000263069

Sites

Active site4741 By similarity
Metal binding4731Zinc; catalytic By similarity
Metal binding4771Zinc; catalytic By similarity
Metal binding4801Zinc; catalytic By similarity

Amino acid modifications

Modified residue2781Phosphotyrosine By similarity
Modified residue5381N6-acetyllysine By similarity

Experimental info

Sequence conflict501C → R in AAI49984. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q1JPJ8 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 70627C332F5A7B8B

FASTA68778,139
        10         20         30         40         50         60 
MKPPAACTGD ALDVVAPCSA VNHLRWDLSA QQIAELTTEL IEQTKRVYDC VGAQEPQDVS 

        70         80         90        100        110        120 
YENTLKALAD VEVSYTVQRN ILDFPQHVSP SKDIRTASTE ADKKLSEFDV EMSMRQDVYQ 

       130        140        150        160        170        180 
RIVWLQEKVQ KDSLRPEASR YLERLIKLGR RNGLHLPEET QEKIKSIKKK LSLLCIDFNK 

       190        200        210        220        230        240 
NLNEDTTFLP FTREELGGLP EDFLNSLEKT EDEKLKVTLK YPHYFPLLKK CHVPETRRKV 

       250        260        270        280        290        300 
EEAFNCRCKE ENCAILRELV RLRAQKSRLL GFSTHADYVL EMNMAKTSQV VATFLDELAQ 

       310        320        330        340        350        360 
KLKPLGEQER AVILELKRAE CEQRGLAFDG RINAWDMRYY MNQVEETRYR VDQNLLKEYF 

       370        380        390        400        410        420 
PMQVVTRGLL GIYQELLGLS FQLEEGAAVW HEDVALYAVR DAASGKLIGK FYLDLYPREG 

       430        440        450        460        470        480 
KYGHAACFGL QPGCLRKDGS RQIAIAAMVA NFTKPTPDAP SLLQHDEVET YFHEFGHVMH 

       490        500        510        520        530        540 
QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEAEPLLR MSQHYRTGSS IPQELLDKLI 

       550        560        570        580        590        600 
KSRQANTGLF NLRQIVLAKV DQALHTQTAA DPAKEYARLC QEILGVPATP GTNMPATFGH 

       610        620        630        640        650        660 
LAGGYDAQYY GYLWSEVYSA DMFHTRFKQE GVLSGKVGMD YRSCILRPGG SEDASVMLKL 

       670        680 
FLGRDPKQDA FLLSKGLQVE GCEPPAC 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Thymus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT025355 mRNA. Translation: ABF57311.1.
BC149983 mRNA. Translation: AAI49984.1.
RefSeqNP_001029163.2. NM_001033991.3.
UniGeneBt.21827.

3D structure databases

ProteinModelPortalQ1JPJ8.
SMRQ1JPJ8. Positions 24-677.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM03.001.

Proteomic databases

PaxDbQ1JPJ8.
PRIDEQ1JPJ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000027246; ENSBTAP00000027246; ENSBTAG00000020446.
GeneID510889.
KEGGbta:510889.

Organism-specific databases

CTD7064.

Phylogenomic databases

eggNOGCOG0339.
GeneTreeENSGT00550000074738.
HOGENOMHOG000245985.
HOVERGENHBG000238.
InParanoidQ1JPJ8.
KOK01392.
OrthoDBEOG7SR4KW.
TreeFamTF300459.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20869665.

Entry information

Entry nameTHOP1_BOVIN
AccessionPrimary (citable) accession number: Q1JPJ8
Secondary accession number(s): A6QQT3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries