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Protein

NADPH-dependent diflavin oxidoreductase 1

Gene

NDOR1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Transfers electrons from NADPH to the Fe/S cluster of CIAPIN1.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotation
  • FMNUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei132 – 1321FMNUniRule annotation
Binding sitei350 – 3501FADUniRule annotation
Binding sitei460 – 4601NADPUniRule annotation
Binding sitei558 – 5581NADPUniRule annotation
Binding sitei596 – 5961FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176FMNUniRule annotation
Nucleotide bindingi59 – 624FMNUniRule annotation
Nucleotide bindingi97 – 10610FMNUniRule annotation
Nucleotide bindingi382 – 3854FADUniRule annotation
Nucleotide bindingi416 – 4194FADUniRule annotation
Nucleotide bindingi515 – 5162NADPUniRule annotation
Nucleotide bindingi521 – 5255NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent diflavin oxidoreductase 1UniRule annotation (EC:1.18.1.-UniRule annotation)
Alternative name(s):
NADPH-dependent FMN and FAD-containing oxidoreductaseUniRule annotation
Gene namesi
Name:NDOR1UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componentsi: Chromosome 11, Unassembled WGS sequence

Subcellular locationi

  • Cytoplasmperinuclear region UniRule annotation

  • Note: Concentrated in perinuclear structure.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597NADPH-dependent diflavin oxidoreductase 1PRO_0000319538Add
BLAST

Proteomic databases

PaxDbiQ1JPJ0.
PRIDEiQ1JPJ0.

Interactioni

Subunit structurei

Interacts with CIAPIN1. Interacts with DCPS.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000054403.

Structurei

3D structure databases

ProteinModelPortaliQ1JPJ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 150145Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini206 – 446241FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG1159. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
HOVERGENiHBG104950.
InParanoidiQ1JPJ0.
OMAiAIPPDYL.
OrthoDBiEOG7QG44B.
TreeFamiTF105716.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03178. NDOR1.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR028879. NDOR1.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1JPJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSARLLVLF GSQTGTAQDV SERLGREARR RQLSCRVEEL DSYPVVNLIN
60 70 80 90 100
EPLVIFVCAT TGQGDPPDNM KSFWRFIFRR SLPSTALRQM DFAVLGLGDS
110 120 130 140 150
SYAKFNFVAK KLHRRLLQLG GSALLPVCLG DDQHELGPDA AIDPWLQDLW
160 170 180 190 200
EKVLGPHPVP LNLDLSPPGV LWPSKFTLQF LKDTPSSGPE ELCAAGTDPQ
210 220 230 240 250
GPPSELQPFL APMVSNQRVT GPSHFQDVRL IEFDISGSGI SFAAGDLVLI
260 270 280 290 300
QPENTASHVQ QFCQALGLDP EQHFTLQPRE PGVTCPTRLP QPCSVRRLVS
310 320 330 340 350
QYLDIASVPR RSFFELLACL SPHELEREKL WEFGSARGQE ELCEYCTRPR
360 370 380 390 400
RTALEVLCDF PHTAAAVPPD YLLDLLPLIR PRAFSIASSL RAHPSRLQIL
410 420 430 440 450
VAVVQYQTRL REPRRGLCSS WLASLDPAQG PVRVPLWVRS GGLTFPKTPD
460 470 480 490 500
VPVIMVGPGT GVAPFRAAIQ ERVAQGETGN VLFFGCRRRD QDFYWEAEWE
510 520 530 540 550
QLQARGCLTL VTAFSREQEQ KVYVQHRLRA LGPLVWELLD GRGAHFYLAG
560 570 580 590
NAKYMPADVC DTLLSIFREE GGLSDPDAAA YLAQLQRTLR FQTETWA
Length:597
Mass (Da):66,568
Last modified:October 16, 2013 - v2
Checksum:i01CA1B51157D58E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti331 – 3311W → R in ABF57319 (PubMed:16305752).Curated
Sequence conflicti398 – 3981Q → H in ABF57319 (PubMed:16305752).Curated
Sequence conflicti542 – 5421R → G in ABF57319 (PubMed:16305752).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025363 mRNA. Translation: ABF57319.1.
DAAA02032411 Genomic DNA. No translation available.
RefSeqiNP_001039623.2. NM_001046158.2.
UniGeneiBt.27888.

Genome annotation databases

EnsembliENSBTAT00000066145; ENSBTAP00000054403; ENSBTAG00000047061.
GeneIDi513873.
KEGGibta:513873.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025363 mRNA. Translation: ABF57319.1.
DAAA02032411 Genomic DNA. No translation available.
RefSeqiNP_001039623.2. NM_001046158.2.
UniGeneiBt.27888.

3D structure databases

ProteinModelPortaliQ1JPJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000054403.

Proteomic databases

PaxDbiQ1JPJ0.
PRIDEiQ1JPJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000066145; ENSBTAP00000054403; ENSBTAG00000047061.
GeneIDi513873.
KEGGibta:513873.

Organism-specific databases

CTDi27158.

Phylogenomic databases

eggNOGiKOG1159. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
HOVERGENiHBG104950.
InParanoidiQ1JPJ0.
OMAiAIPPDYL.
OrthoDBiEOG7QG44B.
TreeFamiTF105716.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03178. NDOR1.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR028879. NDOR1.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-577.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.

Entry informationi

Entry nameiNDOR1_BOVIN
AccessioniPrimary (citable) accession number: Q1JPJ0
Secondary accession number(s): G3MXY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 16, 2013
Last modified: July 6, 2016
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.