Q1JPD6 (STPAP_BOVIN) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 47.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Speckle targeted PIP5K1A-regulated poly(A) polymerase Short name=Star-PAP EC=2.7.7.19 Alternative name(s): RNA-binding motif protein 21 Short name=RNA-binding protein 21 U6 snRNA-specific terminal uridylyltransferase 1 Short name=U6-TUTase EC=2.7.7.52 | ||||
| Gene names |
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| Organism | Bos taurus (Bovine) [Reference proteome] | ||||
| Taxonomic identifier | 9913 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 871 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation By similarity. |
| Catalytic activity | UTP + RNA(n) = diphosphate + RNA(n+1). ATP + RNA(n) = diphosphate + RNA(n+1). |
| Cofactor | Magnesium or manganese By similarity. |
| Enzyme regulation | Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) By similarity. |
| Subunit structure | Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction By similarity. |
| Subcellular location | Nucleus › nucleolus By similarity. Nucleus speckle By similarity. |
| Post-translational modification | Phosphorylated by CK1 in the proline-rich (Pro-rich) region. |
| Sequence similarities | Belongs to the DNA polymerase type-B-like family. Contains 1 C2H2-type zinc finger. Contains 1 PAP-associated domain. Contains 1 RRM (RNA recognition motif) domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 871 | 871 | Speckle targeted PIP5K1A-regulated poly(A) polymerase | PRO_0000254185 | |||||
Regions | |||||||||
| Domain | 56 – 128 | 73 | RRM | ||||||
| Domain | 489 – 547 | 59 | PAP-associated | ||||||
| Zinc finger | 16 – 40 | 25 | C2H2-type | ||||||
| Compositional bias | 229 – 310 | 82 | Pro-rich | ||||||
Sites | |||||||||
| Metal binding | 216 | 1 | Magnesium or manganese; catalytic By similarity | ||||||
| Metal binding | 218 | 1 | Magnesium or manganese; catalytic By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 748 | 1 | Phosphoserine By similarity | ||||||
Sequences
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References
| [1] | "Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BT025417 mRNA. Translation: ABF57373.1. |
| IPI | IPI00705794. |
| RefSeq | NP_001073791.1. NM_001080322.1. |
| UniGene | Bt.21690. |
3D structure databases | |
| ProteinModelPortal | Q1JPD6. |
| SMR | Q1JPD6. Positions 55-140. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 9913.ENSBTAP00000015724. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 616339. |
| KEGG | bta:616339. |
Organism-specific databases | |
| CTD | 64852. |
Phylogenomic databases | |
| eggNOG | COG5260. |
| HOVERGEN | HBG079670. |
| InParanoid | Q1JPD6. |
| OrthoDB | EOG480HWB. |
Family and domain databases | |
| Gene3D | 3.30.70.330. 1 hit. |
| InterPro | IPR012677. Nucleotide-bd_a/b_plait. IPR002058. PAP_assoc. IPR000504. RRM_dom. IPR015880. Znf_C2H2-like. [Graphical view] |
| Pfam | PF03828. PAP_assoc. 1 hit. [Graphical view] |
| SMART | SM00360. RRM. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view] |
| PROSITE | PS50102. RRM. 1 hit. PS00028. ZINC_FINGER_C2H2_1. False negative. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 20900088. |
Entry information
| Entry name | STPAP_BOVIN | ||||||||
| Accession | Primary (citable) accession number: Q1JPD6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |