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Q1JPD6

- STPAP_BOVIN

UniProt

Q1JPD6 - STPAP_BOVIN

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Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

TUT1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation (By similarity).By similarity

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).
ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium or manganese.By similarity

Enzyme regulationi

Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi216 – 2161Magnesium or manganese; catalyticBy similarity
Metal bindingi218 – 2181Magnesium or manganese; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 4025C2H2-typeAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. mRNA 3'-UTR binding Source: UniProtKB
  4. polynucleotide adenylyltransferase activity Source: UniProtKB
  5. RNA binding Source: UniProtKB
  6. RNA uridylyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mRNA cleavage Source: UniProtKB
  2. mRNA polyadenylation Source: UniProtKB
  3. snRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
Short name:
Star-PAP
Alternative name(s):
RNA-binding motif protein 21
Short name:
RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
Short name:
U6-TUTase
Gene namesi
Name:TUT1
Synonyms:RBM21
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Nucleusnucleolus By similarity. Nucleus speckle By similarity

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB
  2. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 871871Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000254185Add
BLAST

Post-translational modificationi

Phosphorylated by CK1 in the proline-rich (Pro-rich) region.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ1JPD6.

Interactioni

Subunit structurei

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015724.

Structurei

3D structure databases

ProteinModelPortaliQ1JPD6.
SMRiQ1JPD6. Positions 55-140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 12873RRMPROSITE-ProRule annotationAdd
BLAST
Domaini489 – 54759PAP-associatedAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi229 – 31082Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated
Contains 1 C2H2-type zinc finger.Curated
Contains 1 PAP-associated domain.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 4025C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5260.
HOVERGENiHBG079670.
InParanoidiQ1JPD6.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1JPD6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAVDSDIEP LPRGGFRCCL CHITTANQPS LDAHLGGRKH RHLVELRATR
60 70 80 90 100
KAQGLRSVFV SGFPRDVDST QLSEYFQAFG PVASVVMDKD KGVFAIVEMG
110 120 130 140 150
DLGAREAVLS QPQHSLGGRR LRVRPREQIE FQSPASRSPK RVAPDSHQLI
160 170 180 190 200
KALAEAPDVE AQMVKLVGLR ELSEAERQLR SLVVALMQEV FAEFFPGCVV
210 220 230 240 250
HPFGSSINSF DVHGCDLDLF LDLGDLDEPQ PAPKAPESPS LDSALASPLD
260 270 280 290 300
PQALACTPAS PPDSQPPASP QDSEALDFEA PSSSLAPRTP DSALASETLA
310 320 330 340 350
SPRSLPPASP LQEDQGDGDQ GKAVELAEAL KGEKAEGGAM LELVGSILRG
360 370 380 390 400
CVPGVYRVQT VPSARCPVVK FCHRPSGLHG DISLSNRLAL HNSRFLSLCS
410 420 430 440 450
ELDGRVRPLV YTLRCWAQGR GLSGSGPLLN NYALTLLVIY FLQTRDPPVL
460 470 480 490 500
PTVSQLTQKA GEQVEVDGWD CSFPRDASRL EPSTNKEPLS SLLAQFFSCV
510 520 530 540 550
SCWDLRGSLL SLREGQALSV AGGLPSNLSE GLRLGPMNLQ DPFDLSHNVA
560 570 580 590 600
ANVTSRVAGR LQNCCRAAAN YCRSLQYQRR SSRGRDWGLL PLLQPSSPSS
610 620 630 640 650
ILSATPIPLP PASFTQLTAV LAQVLREALG CHIEQGTKRL RSEGGGPGEP
660 670 680 690 700
PQGGTSKRAK LDGQKKSCEE GPEEQQGCAG EHGEDGVEEM VIEVGESVQD
710 720 730 740 750
WVMRSPGQLG ELPLMTGKHL ATREEGQSGT AALAKQGPRG PEAACEGSQA
760 770 780 790 800
EAEKRVSLTV SWRCALWHRV WQGRRRARRR LQQQIKEGGG SGAGSGAEWL
810 820 830 840 850
ATEAQVTREL RGLSSTEQRP EAEPLLTFVA STSQADQSLT VTPLQDSQGL
860 870
FPDLHHFLQV FLPQALRNLL K
Length:871
Mass (Da):93,811
Last modified:June 13, 2006 - v1
Checksum:iE681018AFBA18459
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT025417 mRNA. Translation: ABF57373.1.
RefSeqiNP_001073791.1. NM_001080322.1.
UniGeneiBt.21690.

Genome annotation databases

GeneIDi616339.
KEGGibta:616339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT025417 mRNA. Translation: ABF57373.1 .
RefSeqi NP_001073791.1. NM_001080322.1.
UniGenei Bt.21690.

3D structure databases

ProteinModelPortali Q1JPD6.
SMRi Q1JPD6. Positions 55-140.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000015724.

Proteomic databases

PRIDEi Q1JPD6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 616339.
KEGGi bta:616339.

Organism-specific databases

CTDi 64852.

Phylogenomic databases

eggNOGi COG5260.
HOVERGENi HBG079670.
InParanoidi Q1JPD6.

Miscellaneous databases

NextBioi 20900088.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiSTPAP_BOVIN
AccessioniPrimary (citable) accession number: Q1JPD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 13, 2006
Last modified: October 1, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3