Reviewed, UniProtKB/Swiss-Prot Q1JPD6 (TUT1_BOVIN)

Names and origin

Protein names

Recommended name:
    U6 snRNA-specific terminal uridylyltransferase 1
      Short name=U6-TUTase
    EC=2.7.7.52
Alternative name(s):
    RNA-binding motif protein 21
      Short name=RNA-binding protein 21

Gene names

Name:	TUT1
Synonyms:	RBM21

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	871 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Highly specific terminal uridylyltransferase that exclusively accepts U6 snRNA as substrate. U6 snRNA is unique in that nucleotides are both added to and removed from its 3'-end. U6-TUTase is responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA By similarity.
Catalytic activity	UTP + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Nucleus › nucleolus By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA uridylyltransferase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 871	871	U6 snRNA-specific terminal uridylyltransferase 1		PRO_0000254185
Regions
	Domain	56 – 128	73	RRM
Amino acid modifications
	Modified residue	748	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1JPD6-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: E681018AFBA18459	FASTA	871	93,811
	10 20 30 40 50 60 MAAVDSDIEP LPRGGFRCCL CHITTANQPS LDAHLGGRKH RHLVELRATR KAQGLRSVFV 70 80 90 100 110 120 SGFPRDVDST QLSEYFQAFG PVASVVMDKD KGVFAIVEMG DLGAREAVLS QPQHSLGGRR 130 140 150 160 170 180 LRVRPREQIE FQSPASRSPK RVAPDSHQLI KALAEAPDVE AQMVKLVGLR ELSEAERQLR 190 200 210 220 230 240 SLVVALMQEV FAEFFPGCVV HPFGSSINSF DVHGCDLDLF LDLGDLDEPQ PAPKAPESPS 250 260 270 280 290 300 LDSALASPLD PQALACTPAS PPDSQPPASP QDSEALDFEA PSSSLAPRTP DSALASETLA 310 320 330 340 350 360 SPRSLPPASP LQEDQGDGDQ GKAVELAEAL KGEKAEGGAM LELVGSILRG CVPGVYRVQT 370 380 390 400 410 420 VPSARCPVVK FCHRPSGLHG DISLSNRLAL HNSRFLSLCS ELDGRVRPLV YTLRCWAQGR 430 440 450 460 470 480 GLSGSGPLLN NYALTLLVIY FLQTRDPPVL PTVSQLTQKA GEQVEVDGWD CSFPRDASRL 490 500 510 520 530 540 EPSTNKEPLS SLLAQFFSCV SCWDLRGSLL SLREGQALSV AGGLPSNLSE GLRLGPMNLQ 550 560 570 580 590 600 DPFDLSHNVA ANVTSRVAGR LQNCCRAAAN YCRSLQYQRR SSRGRDWGLL PLLQPSSPSS 610 620 630 640 650 660 ILSATPIPLP PASFTQLTAV LAQVLREALG CHIEQGTKRL RSEGGGPGEP PQGGTSKRAK 670 680 690 700 710 720 LDGQKKSCEE GPEEQQGCAG EHGEDGVEEM VIEVGESVQD WVMRSPGQLG ELPLMTGKHL 730 740 750 760 770 780 ATREEGQSGT AALAKQGPRG PEAACEGSQA EAEKRVSLTV SWRCALWHRV WQGRRRARRR 790 800 810 820 830 840 LQQQIKEGGG SGAGSGAEWL ATEAQVTREL RGLSSTEQRP EAEPLLTFVA STSQADQSLT 850 860 870 VTPLQDSQGL FPDLHHFLQV FLPQALRNLL K

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT025417 mRNA. Translation: ABF57373.1.
IPI	IPI00705794.
RefSeq	NP_001073791.1.
UniGene	Bt.21690
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000011846. Bos taurus. [Contig view]
GeneID	616339.
KEGG	bta:616339.
Phylogenomic databases
HOVERGEN	Q1JPD6.
Enzyme and pathway databases
BRENDA	2.7.7.52. 251.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR002058. PAP_assoc. IPR000504. RRM_RNP1. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF03828. PAP_assoc. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TUT1_BOVIN

Accession

Primary (citable) accession number: Q1JPD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	June 13, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)