Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q1JPD6

- STPAP_BOVIN

UniProt

Q1JPD6 - STPAP_BOVIN

Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

TUT1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (13 Jun 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation By similarity.By similarity

    Catalytic activityi

    UTP + RNA(n) = diphosphate + RNA(n+1).
    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Enzyme regulationi

    Adenylyltransferase activity is specifically phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi216 – 2161Magnesium or manganese; catalyticBy similarity
    Metal bindingi218 – 2181Magnesium or manganese; catalyticBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri16 – 4025C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. mRNA 3'-UTR binding Source: UniProtKB
    4. polynucleotide adenylyltransferase activity Source: UniProtKB
    5. RNA binding Source: UniProtKB
    6. RNA uridylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. mRNA cleavage Source: UniProtKB
    2. mRNA polyadenylation Source: UniProtKB
    3. snRNA processing Source: UniProtKB

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
    Short name:
    Star-PAP
    Alternative name(s):
    RNA-binding motif protein 21
    Short name:
    RNA-binding protein 21
    U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
    Short name:
    U6-TUTase
    Gene namesi
    Name:TUT1
    Synonyms:RBM21
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Nucleusnucleolus By similarity. Nucleus speckle By similarity

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB
    2. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 871871Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000254185Add
    BLAST

    Post-translational modificationi

    Phosphorylated by CK1 in the proline-rich (Pro-rich) region.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ1JPD6.

    Interactioni

    Subunit structurei

    Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000015724.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1JPD6.
    SMRiQ1JPD6. Positions 55-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 12873RRMPROSITE-ProRule annotationAdd
    BLAST
    Domaini489 – 54759PAP-associatedAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi229 – 31082Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B-like family.Curated
    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 PAP-associated domain.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri16 – 4025C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5260.
    HOVERGENiHBG079670.
    InParanoidiQ1JPD6.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1JPD6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAVDSDIEP LPRGGFRCCL CHITTANQPS LDAHLGGRKH RHLVELRATR    50
    KAQGLRSVFV SGFPRDVDST QLSEYFQAFG PVASVVMDKD KGVFAIVEMG 100
    DLGAREAVLS QPQHSLGGRR LRVRPREQIE FQSPASRSPK RVAPDSHQLI 150
    KALAEAPDVE AQMVKLVGLR ELSEAERQLR SLVVALMQEV FAEFFPGCVV 200
    HPFGSSINSF DVHGCDLDLF LDLGDLDEPQ PAPKAPESPS LDSALASPLD 250
    PQALACTPAS PPDSQPPASP QDSEALDFEA PSSSLAPRTP DSALASETLA 300
    SPRSLPPASP LQEDQGDGDQ GKAVELAEAL KGEKAEGGAM LELVGSILRG 350
    CVPGVYRVQT VPSARCPVVK FCHRPSGLHG DISLSNRLAL HNSRFLSLCS 400
    ELDGRVRPLV YTLRCWAQGR GLSGSGPLLN NYALTLLVIY FLQTRDPPVL 450
    PTVSQLTQKA GEQVEVDGWD CSFPRDASRL EPSTNKEPLS SLLAQFFSCV 500
    SCWDLRGSLL SLREGQALSV AGGLPSNLSE GLRLGPMNLQ DPFDLSHNVA 550
    ANVTSRVAGR LQNCCRAAAN YCRSLQYQRR SSRGRDWGLL PLLQPSSPSS 600
    ILSATPIPLP PASFTQLTAV LAQVLREALG CHIEQGTKRL RSEGGGPGEP 650
    PQGGTSKRAK LDGQKKSCEE GPEEQQGCAG EHGEDGVEEM VIEVGESVQD 700
    WVMRSPGQLG ELPLMTGKHL ATREEGQSGT AALAKQGPRG PEAACEGSQA 750
    EAEKRVSLTV SWRCALWHRV WQGRRRARRR LQQQIKEGGG SGAGSGAEWL 800
    ATEAQVTREL RGLSSTEQRP EAEPLLTFVA STSQADQSLT VTPLQDSQGL 850
    FPDLHHFLQV FLPQALRNLL K 871
    Length:871
    Mass (Da):93,811
    Last modified:June 13, 2006 - v1
    Checksum:iE681018AFBA18459
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT025417 mRNA. Translation: ABF57373.1.
    RefSeqiNP_001073791.1. NM_001080322.1.
    UniGeneiBt.21690.

    Genome annotation databases

    GeneIDi616339.
    KEGGibta:616339.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT025417 mRNA. Translation: ABF57373.1 .
    RefSeqi NP_001073791.1. NM_001080322.1.
    UniGenei Bt.21690.

    3D structure databases

    ProteinModelPortali Q1JPD6.
    SMRi Q1JPD6. Positions 55-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000015724.

    Proteomic databases

    PRIDEi Q1JPD6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 616339.
    KEGGi bta:616339.

    Organism-specific databases

    CTDi 64852.

    Phylogenomic databases

    eggNOGi COG5260.
    HOVERGENi HBG079670.
    InParanoidi Q1JPD6.

    Miscellaneous databases

    NextBioi 20900088.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiSTPAP_BOVIN
    AccessioniPrimary (citable) accession number: Q1JPD6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3