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Q1JKZ4

- Q1JKZ4_STRPC

UniProt

Q1JKZ4 - Q1JKZ4_STRPC

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus pyogenes serotype M12 (strain MGAS9429)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO370551:GHLY-1297-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
Ordered Locus Names:MGAS9429_Spy1238Imported
OrganismiStreptococcus pyogenes serotype M12 (strain MGAS9429)Imported
Taxonomic identifieri370551 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002433: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi370551.MGAS9429_Spy1238.

Structurei

3D structure databases

ProteinModelPortaliQ1JKZ4.
SMRiQ1JKZ4. Positions 1-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1JKZ4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLKETLNLG KTAFPMRAGL PNKEPQWQAA WEQAELYKKR QELNAGKPAF
60 70 80 90 100
HLHDGPPYAN GNIHVGHALN KISKDIIVRS KSMSGFQAPY VPGWDTHGLP
110 120 130 140 150
IEQVLAKQGI KRKEMDLAEY LEMCRQYALS QVDKQRDDFK RLGVSADWEN
160 170 180 190 200
PYVTLDPQFE ADQIRVFGAM AEKGYIYRGA KPVYWSWSSE SALAEAEIEY
210 220 230 240 250
HDIDSTSLYY ANKVKDGKGI LDTNTYIVVW TTTPFTVTAS RGLTVGPDMD
260 270 280 290 300
YLVVKPAGSD RQYVVAEGLL DSLAGKFGWE SFETLASHKG ADLEYIVTEH
310 320 330 340 350
PWDTDVEELV ILGDHVTLES GTGIVHTAPG FGEDDYNVGT KYKLEVAVTV
360 370 380 390 400
DERGLMMENA GPDFHGQFYN KVTPIVIDKL GDLLLAQEVI NHSYPFDWRT
410 420 430 440 450
KKPIIWRAVP QWFASVSDFR QDILDEIEKT TFHPSWGETR LYNMIRDRGD
460 470 480 490 500
WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHVADLFQEN GSIIWWQKEA
510 520 530 540 550
KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSWNGVMN ARENLSYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AILSQGFVLD GKGEKMSKSK
610 620 630 640 650
GNIISPNDVA KQYGADILRL WVASVDTDND IRVSMEILGQ VSETYRKIRN
660 670 680 690 700
TLRFLIANTS DFNPATDTVA YADLGAVDKY MTIVFNQLVA TITDAYERYD
710 720 730 740 750
FMAIYKAVVN FVTVDLSAFY LDFAKDVVYI EAANSLERRR MQTVFYDILV
760 770 780 790 800
KITKLLTPIL PHTTEEIWSY LEYESEAFVQ LAEMPVAETF SAQEDILEAW
810 820 830 840 850
SAFMTLRTQA QKALEEARNA KIIGKSLEAH LTIYASEEVK TLLTALDSDI
860 870 880 890 900
ALLLIVSQLT IADLADAPAD AVAFEGVAFM VEHAIGEVCE RSRRIDPTTR
910 920 930
MRSYNAFVCD HSAKIIEENF PEAVAEGFEE SGK
Length:933
Mass (Da):105,065
Last modified:June 13, 2006 - v1
Checksum:i22001A131679F834
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000259 Genomic DNA. Translation: ABF32425.1.
RefSeqiYP_596969.1. NC_008021.1.

Genome annotation databases

EnsemblBacteriaiABF32425; ABF32425; MGAS9429_Spy1238.
GeneIDi4061215.
KEGGispk:MGAS9429_Spy1238.
PATRICi19753527. VBIStrPyo37061_1253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000259 Genomic DNA. Translation: ABF32425.1 .
RefSeqi YP_596969.1. NC_008021.1.

3D structure databases

ProteinModelPortali Q1JKZ4.
SMRi Q1JKZ4. Positions 1-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 370551.MGAS9429_Spy1238.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABF32425 ; ABF32425 ; MGAS9429_Spy1238 .
GeneIDi 4061215.
KEGGi spk:MGAS9429_Spy1238.
PATRICi 19753527. VBIStrPyo37061_1253.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPYO370551:GHLY-1297-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus."
    Beres S.B., Richter E.W., Nagiec M.J., Sumby P., Porcella S.F., DeLeo F.R., Musser J.M.
    Proc. Natl. Acad. Sci. U.S.A. 103:7059-7064(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS9429Imported.

Entry informationi

Entry nameiQ1JKZ4_STRPC
AccessioniPrimary (citable) accession number: Q1JKZ4
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2006
Last sequence update: June 13, 2006
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3