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Q1JKZ4

- Q1JKZ4_STRPC

UniProt

Q1JKZ4 - Q1JKZ4_STRPC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus pyogenes serotype M12 (strain MGAS9429)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
    Binding sitei598 – 5981ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSPYO370551:GHLY-1297-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:ileSUniRule annotationImported
    Ordered Locus Names:MGAS9429_Spy1238Imported
    OrganismiStreptococcus pyogenes serotype M12 (strain MGAS9429)Imported
    Taxonomic identifieri370551 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000002433: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi370551.MGAS9429_Spy1238.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1JKZ4.
    SMRiQ1JKZ4. Positions 1-918.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionUniRule annotationAdd
    BLAST
    Motifi595 – 5995"KMSKS" regionUniRule annotation

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1JKZ4-1 [UniParc]FASTAAdd to Basket

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    MKLKETLNLG KTAFPMRAGL PNKEPQWQAA WEQAELYKKR QELNAGKPAF    50
    HLHDGPPYAN GNIHVGHALN KISKDIIVRS KSMSGFQAPY VPGWDTHGLP 100
    IEQVLAKQGI KRKEMDLAEY LEMCRQYALS QVDKQRDDFK RLGVSADWEN 150
    PYVTLDPQFE ADQIRVFGAM AEKGYIYRGA KPVYWSWSSE SALAEAEIEY 200
    HDIDSTSLYY ANKVKDGKGI LDTNTYIVVW TTTPFTVTAS RGLTVGPDMD 250
    YLVVKPAGSD RQYVVAEGLL DSLAGKFGWE SFETLASHKG ADLEYIVTEH 300
    PWDTDVEELV ILGDHVTLES GTGIVHTAPG FGEDDYNVGT KYKLEVAVTV 350
    DERGLMMENA GPDFHGQFYN KVTPIVIDKL GDLLLAQEVI NHSYPFDWRT 400
    KKPIIWRAVP QWFASVSDFR QDILDEIEKT TFHPSWGETR LYNMIRDRGD 450
    WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHVADLFQEN GSIIWWQKEA 500
    KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSWNGVMN ARENLSYPAD 550
    LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AILSQGFVLD GKGEKMSKSK 600
    GNIISPNDVA KQYGADILRL WVASVDTDND IRVSMEILGQ VSETYRKIRN 650
    TLRFLIANTS DFNPATDTVA YADLGAVDKY MTIVFNQLVA TITDAYERYD 700
    FMAIYKAVVN FVTVDLSAFY LDFAKDVVYI EAANSLERRR MQTVFYDILV 750
    KITKLLTPIL PHTTEEIWSY LEYESEAFVQ LAEMPVAETF SAQEDILEAW 800
    SAFMTLRTQA QKALEEARNA KIIGKSLEAH LTIYASEEVK TLLTALDSDI 850
    ALLLIVSQLT IADLADAPAD AVAFEGVAFM VEHAIGEVCE RSRRIDPTTR 900
    MRSYNAFVCD HSAKIIEENF PEAVAEGFEE SGK 933
    Length:933
    Mass (Da):105,065
    Last modified:June 13, 2006 - v1
    Checksum:i22001A131679F834
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000259 Genomic DNA. Translation: ABF32425.1.
    RefSeqiYP_596969.1. NC_008021.1.

    Genome annotation databases

    EnsemblBacteriaiABF32425; ABF32425; MGAS9429_Spy1238.
    GeneIDi4061215.
    KEGGispk:MGAS9429_Spy1238.
    PATRICi19753527. VBIStrPyo37061_1253.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000259 Genomic DNA. Translation: ABF32425.1 .
    RefSeqi YP_596969.1. NC_008021.1.

    3D structure databases

    ProteinModelPortali Q1JKZ4.
    SMRi Q1JKZ4. Positions 1-918.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 370551.MGAS9429_Spy1238.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABF32425 ; ABF32425 ; MGAS9429_Spy1238 .
    GeneIDi 4061215.
    KEGGi spk:MGAS9429_Spy1238.
    PATRICi 19753527. VBIStrPyo37061_1253.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SPYO370551:GHLY-1297-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus."
      Beres S.B., Richter E.W., Nagiec M.J., Sumby P., Porcella S.F., DeLeo F.R., Musser J.M.
      Proc. Natl. Acad. Sci. U.S.A. 103:7059-7064(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MGAS9429Imported.

    Entry informationi

    Entry nameiQ1JKZ4_STRPC
    AccessioniPrimary (citable) accession number: Q1JKZ4
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 13, 2006
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3