Reviewed, UniProtKB/Swiss-Prot Q1JKW9 (ASNA_STRPC)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	MGAS9429_Spy1263

Organism

Streptococcus pyogenes serotype M12 (strain MGAS9429) [Complete proteome] [HAMAP]

Taxonomic identifier

370551 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017966

Sequences

	Sequence		Length	Mass (Da)
	Q1JKW9-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 255B344AB733920F	FASTA	330	37,399
	10 20 30 40 50 60 MKKSFIHQQE EISFVKNTFT QYLIAKLDVV EVQGPILSRV GDGMQDNLSG TENPVSVNVL 70 80 90 100 110 120 KIPNATFEVV HSLAKWKRHT LARFGFNEGE GLVVNMKALR PDEDSLDQTH SVYVDQWDWE 130 140 150 160 170 180 KVIPDGKRNL AYLKETVETI YKVIRLTELA VEARYDIEAV LPKKITFIHT EELVAKYPDL 190 200 210 220 230 240 TPKERENAIT KEFGAVFLIG IGGVLPDGKP HDGRAPDYDD WTTETENGYH GLNGDILVWN 250 260 270 280 290 300 DQLGSAFELS SMGIRVDEEA LKRQVEMTGD QDRLAFDWHK SLLNGLFPLT IGGGIGQSRM 310 320 330 VMFLLRKKHI GEVQTSVWPQ EVRDSYDNIL

References

[1]

"Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus." Beres S.B., Richter E.W., Nagiec M.J., Sumby P., Porcella S.F., DeLeo F.R., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 103:7059-7064(2006) [PubMed: 16636287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000259 Genomic DNA. Translation: ABF32450.1.
RefSeq	YP_596994.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4061919.
GenomeReviews	Gene locus MGAS9429_Spy1263 in contig CP000259_GR.
KEGG	spk:MGAS9429_Spy1263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1JKW9.
OMA	Q1JKW9. LNDNLNG.
Enzyme and pathway databases
BioCyc	SPYO370551:MGAS9429_SPY1263-MON.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_STRPC

Accession

Primary (citable) accession number: Q1JKW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)