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Protein

Glutamate 5-kinase

Gene

proB

Organism
Streptococcus pyogenes serotype M12 (strain MGAS9429)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathway: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171ATPUniRule annotation
Binding sitei57 – 571SubstrateUniRule annotation
Binding sitei144 – 1441SubstrateUniRule annotation
Binding sitei160 – 1601Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi180 – 1812ATPUniRule annotation
Nucleotide bindingi222 – 2287ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO370551:GHLY-1440-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:MGAS9429_Spy1369
OrganismiStreptococcus pyogenes serotype M12 (strain MGAS9429)
Taxonomic identifieri370551 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002433 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Glutamate 5-kinasePRO_0000253005Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ1JKL3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiWIAHSEG.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1JKL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMMKRQFED VTRIVIKIGT SSLVLPTGKI NLEKIDQLAF VISSLMNKGK
60 70 80 90 100
EVILVSSGAM GFGLDILKME KRPTNLAKQQ AVSSVGQVAM MSLYSQIFAH
110 120 130 140 150
YQTNVSQILL TRDVVVFPES LANVTNAFES LISLGIVPIV NENDAVSVDE
160 170 180 190 200
MDHATKFGDN DRLSAVVAGI TKADLLIMLS DIDGLFDKNP TIYEDAQLRS
210 220 230 240 250
HVAVITQEII ASAGGAGSKF GTGGMLSKIQ SAQMVFENKG QMVLMNGANP
260 270
RDILRVLEGQ PLGTWFKQIE EVTHD
Length:275
Mass (Da):29,863
Last modified:June 13, 2006 - v1
Checksum:iCDD34990A2C098F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000259 Genomic DNA. Translation: ABF32556.1.
RefSeqiWP_011285062.1. NC_008021.1.
YP_597100.1. NC_008021.1.

Genome annotation databases

EnsemblBacteriaiABF32556; ABF32556; MGAS9429_Spy1369.
KEGGispk:MGAS9429_Spy1369.
PATRICi19753814. VBIStrPyo37061_1384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000259 Genomic DNA. Translation: ABF32556.1.
RefSeqiWP_011285062.1. NC_008021.1.
YP_597100.1. NC_008021.1.

3D structure databases

ProteinModelPortaliQ1JKL3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF32556; ABF32556; MGAS9429_Spy1369.
KEGGispk:MGAS9429_Spy1369.
PATRICi19753814. VBIStrPyo37061_1384.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiWIAHSEG.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciSPYO370551:GHLY-1440-MONOMER.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus."
    Beres S.B., Richter E.W., Nagiec M.J., Sumby P., Porcella S.F., DeLeo F.R., Musser J.M.
    Proc. Natl. Acad. Sci. U.S.A. 103:7059-7064(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS9429.

Entry informationi

Entry nameiPROB_STRPC
AccessioniPrimary (citable) accession number: Q1JKL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 13, 2006
Last modified: June 24, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.