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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Streptococcus pyogenes serotype M2 (strain MGAS10270)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
Active sitei127 – 1271Proton acceptorUniRule annotation
Binding sitei154 – 1541Allosteric activator ADPUniRule annotation
Binding sitei162 – 1621Substrate; shared with dimeric partnerUniRule annotation
Binding sitei212 – 2121Allosteric activator ADPUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation
Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATPUniRule annotation
Nucleotide bindingi102 – 1054ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO370552:GHYF-1158-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:MGAS10270_Spy1103
OrganismiStreptococcus pyogenes serotype M2 (strain MGAS10270)
Taxonomic identifieri370552 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002436 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337ATP-dependent 6-phosphofructokinasePRO_1000059797Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi370552.MGAS10270_Spy1103.

Structurei

3D structure databases

ProteinModelPortaliQ1JGH6.
SMRiQ1JGH6. Positions 1-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni125 – 1273Substrate bindingUniRule annotation
Regioni169 – 1713Substrate bindingUniRule annotation
Regioni185 – 1873Allosteric activator ADP bindingUniRule annotation
Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
Regioni251 – 2544Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiVHHDIVD.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1JGH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIAVLTSG GDAPGMNAAI RAVVRKAISE GMEVYGINRG YAGMVDGDIF
60 70 80 90 100
PLGSKEVGDK ISRGGTFLYS ARYPEFAQLE GQLAGIEQLK KHGIEGVVVI
110 120 130 140 150
GGDGSYHGAM RLTEHGFPAV GIPGTIDNDI AGTDYTIGFD TAVNTAVEAI
160 170 180 190 200
DKLRDTSSSH GRTFVVEVMG RNAGDIALWA GIASGADQII VPEEEFDIEK
210 220 230 240 250
VASTIQYDFE HKGKNHHIIV LAEGVMSGEA FAQKLKEAGD KSDLRVTNLG
260 270 280 290 300
HILRGGSPTA RDRVIASWMG SHAVELLKEG KGGLAVGIHN EELVESPILG
310 320 330
TAEEGALFSL TEEGKIIVNN PHKARLDFAA LNRSLSQ
Length:337
Mass (Da):35,762
Last modified:June 13, 2006 - v1
Checksum:iDA99570A8BF391FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000260 Genomic DNA. Translation: ABF34168.1.
RefSeqiYP_598712.1. NC_008022.1.

Genome annotation databases

EnsemblBacteriaiABF34168; ABF34168; MGAS10270_Spy1103.
GeneIDi4064426.
KEGGisph:MGAS10270_Spy1103.
PATRICi19720128. VBIStrPyo120482_1121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000260 Genomic DNA. Translation: ABF34168.1.
RefSeqiYP_598712.1. NC_008022.1.

3D structure databases

ProteinModelPortaliQ1JGH6.
SMRiQ1JGH6. Positions 1-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi370552.MGAS10270_Spy1103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF34168; ABF34168; MGAS10270_Spy1103.
GeneIDi4064426.
KEGGisph:MGAS10270_Spy1103.
PATRICi19720128. VBIStrPyo120482_1121.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiVHHDIVD.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciSPYO370552:GHYF-1158-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus."
    Beres S.B., Richter E.W., Nagiec M.J., Sumby P., Porcella S.F., DeLeo F.R., Musser J.M.
    Proc. Natl. Acad. Sci. U.S.A. 103:7059-7064(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS10270.

Entry informationi

Entry nameiPFKA_STRPD
AccessioniPrimary (citable) accession number: Q1JGH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 13, 2006
Last modified: January 7, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.