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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Streptococcus pyogenes serotype M12 (strain MGAS2096)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi288Magnesium or manganese 1UniRule annotation1
Metal bindingi301Magnesium or manganese 1UniRule annotation1
Metal bindingi301Magnesium or manganese 2UniRule annotation1
Metal bindingi303Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 217ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:MGAS2096_Spy1229
OrganismiStreptococcus pyogenes serotype M12 (strain MGAS2096)
Taxonomic identifieri370553 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000305001 – 348D-alanine--D-alanine ligaseAdd BLAST348

Structurei

3D structure databases

ProteinModelPortaliQ1JAX7
SMRiQ1JAX7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 334ATP-graspUniRule annotationAdd BLAST203

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000011593
KOiK01921
OMAiYETKYTE
OrthoDBiPOG091H06GK

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q1JAX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQTLVLLY GGRSAEREVS VLSAESVMRA VNYDKFLVKT YFITQMGQFI
60 70 80 90 100
KTQQFSEKPS ESERLMTNET IELTQKIKPS DIYEEGAVVF PVLHGPMGED
110 120 130 140 150
GSIQGFLEVL RMPYIGTNVM SSSIAMDKIT TKRVLESIGI PQVAYTVYID
160 170 180 190 200
GQDLEACLVE TLARLTFPIF VKPANMGSSV GISKAQTKVE LRKAIQLALT
210 220 230 240 250
YDSRVLIEQG VVAREIEVGL LGNDKVKSTL PGEVIKDVDF YDYQAKYVDN
260 270 280 290 300
KITMAIPADV DQSIVTEMRS YAEVAFKALG GCGLSRCDFF LTQDGQVYLN
310 320 330 340
ELNTMPGFTQ WSMYPLLWEN MGLAYPDLIE ELVTLAQEMF DQRESHLI
Length:348
Mass (Da):38,969
Last modified:June 13, 2006 - v1
Checksum:iBC221F78EBD31314
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000261 Genomic DNA Translation: ABF36281.1

Genome annotation databases

EnsemblBacteriaiABF36281; ABF36281; MGAS2096_Spy1229
KEGGispj:MGAS2096_Spy1229

Similar proteinsi

Entry informationi

Entry nameiDDL_STRPB
AccessioniPrimary (citable) accession number: Q1JAX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 13, 2006
Last modified: March 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health