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Protein

Glutamate 5-kinase

Gene

proB

Organism
Streptococcus pyogenes serotype M12 (strain MGAS2096)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17ATPUniRule annotation1
Binding sitei57SubstrateUniRule annotation1
Binding sitei144SubstrateUniRule annotation1
Binding sitei160Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 181ATPUniRule annotation2
Nucleotide bindingi222 – 228ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:MGAS2096_Spy1394
OrganismiStreptococcus pyogenes serotype M12 (strain MGAS2096)
Taxonomic identifieri370553 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002530041 – 275Glutamate 5-kinaseAdd BLAST275

Proteomic databases

PRIDEiQ1JAG2

Structurei

3D structure databases

ProteinModelPortaliQ1JAG2
SMRiQ1JAG2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246368
KOiK00931
OMAiNMINCYE
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q1JAG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMMKRQFED VTRIVIKIGT SSLVLPTGKI NLEKIDQLAF VISSLMNKGK
60 70 80 90 100
EVILVSSGAM GFGLDILKME KRPTNLAKQQ AVSSVGQVAM MSLYSQIFAH
110 120 130 140 150
YQTNVSQILL TRDVVVFPES LANVTNAFES LISLGIVPIV NENDAVSVDE
160 170 180 190 200
MDHATKFGDN DRLSAVVAGI TKADLLIMLS DIDGLFDKNP TIYEDAQLRS
210 220 230 240 250
HVAVITQEII ASAGGAGSKF GTGGMLSKIQ SAQMVFENKG QMVLMNGANP
260 270
RDILRVLEGQ PLGTWFKQIE EVTHD
Length:275
Mass (Da):29,863
Last modified:June 13, 2006 - v1
Checksum:iCDD34990A2C098F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000261 Genomic DNA Translation: ABF36446.1

Genome annotation databases

EnsemblBacteriaiABF36446; ABF36446; MGAS2096_Spy1394
KEGGispj:MGAS2096_Spy1394

Similar proteinsi

Entry informationi

Entry nameiPROB_STRPB
AccessioniPrimary (citable) accession number: Q1JAG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 13, 2006
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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