Q1J201 (ISPE_DEIGD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Dgeo_0180

Organism

Deinococcus geothermalis (strain DSM 11300) [Complete proteome] [HAMAP]

Taxonomic identifier

319795 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335709
Regions
	Nucleotide binding	115 – 125	11	ATP Potential
Sites
	Active site	33	1	By similarity
	Active site	154	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1J201 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 6E2E5E3F63A953EC	FASTA	299	30,704
	10 20 30 40 50 60 MPPECGGPPM PLDPLTLRPL APSETATYLA PAKVNLGLSV RGLRTDGYHE LHSVMVPLVV 70 80 90 100 110 120 GDELEIAAAD TLTLRVEGAA LPTDERNLVY RAARAYLDAA GVSGGATITL RKRLPLASGL 130 140 150 160 170 180 GGGSSDAATT LMALARLFPA PVNLPALALT LGADVPFFLL GQAALAQGIG DVLTPLPVPQ 190 200 210 220 230 240 VPLVLVNPGV EVSARDAYAW LDEEEAFTPP LDVEGLLAAL TAQHELPTFN ALQGPVAARH 250 260 270 280 290 APIQAALAAL SSAGLRSPLM SGSGATCFAL AASDAQAHAA AQALQAQHPA WWVVATRTL

References

[1]

"Complete sequence of chromosome 1 of Deinococcus geothermalis DSM 11300." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Daly M.J., Fredrickson J.K., Makarova K.S., Gaidamakova E.K., Zhai M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 11300.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000359 Genomic DNA. Translation: ABF44483.1.
RefSeq	YP_603652.1. NC_008025.1.
3D structure databases
ProteinModelPortal	Q1J201.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1J201.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4058426.
GenomeReviews	Gene locus Dgeo_0180 in contig CP000359_GR.
KEGG	dge:Dgeo_0180.
NMPDR	fig|68909.1.peg.1125.
PATRIC	21622147. VBIDeiGeo41128_0730.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	CQLARMS.
PhylomeDB	Q1J201.
ProtClustDB	PRK14612.
Enzyme and pathway databases
BioCyc	DGEO319795:DGEO_0180-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_DEIGD

Accession

Primary (citable) accession number: Q1J201

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 13, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program