Reviewed, UniProtKB/Swiss-Prot Q1IZQ3 (GLGB_DEIGD)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Dgeo_0981

Organism

Deinococcus geothermalis (strain DSM 11300) [Complete proteome] [HAMAP]

Taxonomic identifier

319795 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 652	652	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000260650
Sites
	Active site	217	1	By similarity
	Active site	252	1	By similarity
	Active site	257	1	By similarity
	Active site	320	1	By similarity
	Active site	322	1	By similarity
	Active site	373	1	By similarity
	Active site	439	1	By similarity
	Active site	440	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IZQ3-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 4E6C502E4EE2D41E	FASTA	652	74,437
	10 20 30 40 50 60 MLDPLRASSP ATLALMSSLP LPLDHGHLQK LVTADLVRPD HLLGAHPVTE NGVEGVRFAV 70 80 90 100 110 120 WAPRAQHVSV VGDFNGWNGF DHPMQRLDFG FWGAFVPSAR PGQRYKFRVT GADGRTVDKM 130 140 150 160 170 180 DPYGAFTEVR PNTASIIWPL AFEWTDDAWM QKRTPGFDRP ISIYEVHVGS WARRDDGWFL 190 200 210 220 230 240 NYRELAHRLA DYVTFMGYTH VELLGVMEHP FDGSWGYQVT GYYAPTSRLG APEDFAYLVN 250 260 270 280 290 300 HLHERGIGVI VDWVPGHFPT DEAGLAHFDG APLYEYSDPR KGYHYDWNTY IFDYGRNEVM 310 320 330 340 350 360 MFLIGSALKW LQDFHVDGLR VDAVASMLYL DFSRTEWVPN IHGGRENLEA IAFLKRLNEV 370 380 390 400 410 420 VHHMAPGCMM IAEESTAFPG VTSPTPFGLG FDYKWAMGWM NDTLYYFEQD PLWRKYHHHK 430 440 450 460 470 480 LTFFNVYRTG ENYILAISHD EVVHLKKAMV SKMPGDWYMQ RAGYRAFLAM MWTTPGKKLL 490 500 510 520 530 540 FMGQEFAQGT EWNHDEALPW DVTDLPEHRG VMNLVRRLNG LYRERPDLHI GDTWEEGQLW 550 560 570 580 590 600 VSADDTDNSV YAYIRRDPRP QAEGGGAWSL AVANLTPVYR EGYPIGVPQG GEYRVLLSTD 610 620 630 640 650 DGEYGGFGTQ QPDLTAKEEG WNGQTHHLRL NLPPMSVLLL EHVGVTPRSE DR

References

[1]

"Complete sequence of chromosome 1 of Deinococcus geothermalis DSM 11300." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Daly M.J., Fredrickson J.K., Makarova K.S., Gaidamakova E.K., Zhai M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000359 Genomic DNA. Translation: ABF45281.1.
RefSeq	YP_604450.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IZQ3.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	4058678.
GenomeReviews	Gene locus Dgeo_0981 in contig CP000359_GR.
KEGG	dge:Dgeo_0981.
NMPDR	fig|68909.1.peg.2034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	PKTASIV.
PhylomeDB	Q1IZQ3.
Enzyme and pathway databases
BioCyc	DGEO319795:DGEO_0981-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_DEIGD

Accession

Primary (citable) accession number: Q1IZQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	June 13, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)