Reviewed, UniProtKB/Swiss-Prot Q1IZL1 (GLUQ_DEIGD)

Names and origin

Protein names

Recommended name:
    Glutamyl-Q tRNA(Asp) synthetase
      Short name=Glu-Q-RSs
    EC=6.1.1.-

Gene names

Name:	gluQ
Ordered Locus Names:	Dgeo_1023

Organism

Deinococcus geothermalis (strain DSM 11300) [Complete proteome] [HAMAP]

Taxonomic identifier

319795 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon By similarity. HAMAP MF_01428
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01428
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Glutamyl-Q tRNA(Asp) synthetase HAMAP MF_01428		PRO_1000024354
Regions
	Region	9 – 13	5	Glutamate binding By similarity
	Motif	12 – 22	11	"HIGH" region HAMAP MF_01428
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_01428
Sites
	Metal binding	100	1	Zinc By similarity
	Metal binding	102	1	Zinc By similarity
	Metal binding	125	1	Zinc By similarity
	Metal binding	129	1	Zinc By similarity
	Binding site	45	1	Glutamate By similarity
	Binding site	184	1	Glutamate By similarity
	Binding site	202	1	Glutamate By similarity
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IZL1-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 72C0BC1B26CD2F21	FASTA	303	33,082
	10 20 30 40 50 60 MNVLPVVGRF APSPTGAMHL GNARTALLAW LHSRALGGLH LLRFEDLDTG RVRSWAYDTT 70 80 90 100 110 120 RRDLEWLGLD WDAEFRQSER LPVYADAVSR LTAAGETYPC TCTRREIRQA IEDSAGAPHG 130 140 150 160 170 180 HEPVYPGTCR LHGAMPGRPA ALRWHVPDGT VCVTDALTCA TLCQNLPAEV GDFVLRRNDG 190 200 210 220 230 240 VYAYHLAVVV DDALMGVTDV VRGADLWTAT PRQVALGRAL GSAPPRYLHV PLMTDFRGER 250 260 270 280 290 300 LAKRGGAPPL RALREGGEEA GRVLSALARS LGWAVPERVS AAELLPLWRA QLTRAGFFIN PQS

References

[1]

"Complete sequence of chromosome 1 of Deinococcus geothermalis DSM 11300." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Daly M.J., Fredrickson J.K., Makarova K.S., Gaidamakova E.K., Zhai M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000359 Genomic DNA. Translation: ABF45323.1.
RefSeq	YP_604492.1.
3D structure databases
SMR	Q1IZL1. Positions 5-297.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IZL1.
Genome annotation databases
GeneID	4057984.
GenomeReviews	Gene locus Dgeo_1023 in contig CP000359_GR.
KEGG	dge:Dgeo_1023.
NMPDR	fig|68909.1.peg.500.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DAPSSYH.
PhylomeDB	Q1IZL1.
Enzyme and pathway databases
BioCyc	DGEO319795:DGEO_1023-MONOMER.
Family and domain databases
HAMAP	MF_01428. Glu_Q_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLUQ_DEIGD

Accession

Primary (citable) accession number: Q1IZL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	January 19, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)