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Q1IZD5 (Q1IZD5_DEIGD) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003
Ordered Locus Names:Dgeo_1101
OrganismDeinococcus geothermalis (strain DSM 11300) [Complete proteome] [HAMAP]
Taxonomic identifier319795 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length1091 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
Q1IZD5 [UniParc].

Last modified June 13, 2006. Version 1.
Checksum: 186387C43FC2BBF9

FASTA1,091122,774
        10         20         30         40         50         60 
MTTKTQASPL FQPVGSQPSF RELEAGILNF WQENRIFEQT QERQDGQPEF VFYEGPPTAN 

        70         80         90        100        110        120 
GRPALHHVLA RSFKDLFPRY KVMQGYFVTR KGGWDTHGLP VEISVEKKLG LLGRNHGASR 

       130        140        150        160        170        180 
EELEEFNRLC RASVWETIQE WNTFTQRLGY WVDLSDPYIT YENEYVESVW NLLKRLHAKG 

       190        200        210        220        230        240 
LIAQDYKVVP LSPRISTTLS RAELGEVDSY RMVDDPSVYV RFPVIWDTLP ERARAALATL 

       250        260        270        280        290        300 
DEADRQDLAL VVWTTTPWTL PSNTLAAVNP DLTYVVARSG LGPVIVAADA TRRLSELHKH 

       310        320        330        340        350        360 
WAPLEVLTSF PGRDLEGVEY EPPFPEVATE LGVLRELHER NAEGRPVMHF VALADFVSAA 

       370        380        390        400        410        420 
DGSGVAHEAP AYGAEDLELA RRYEVPLLFG VDDHGILQVT KERGKFFKDA DKGLIADLKA 

       430        440        450        460        470        480 
RGLMFHAGTI RHRYPFHDRT GDPILYFAKK GWYIRTNSVA DRMLETNEEI NWVPPGIKHG 

       490        500        510        520        530        540 
RFGHWLEGNV DWAISRERYW GTPLPFWMSE DGDLQVVGSV AELSALTGRD LTGLDLHRPS 

       550        560        570        580        590        600 
IDDVTFTRDG KTYRRVPEVL DVWFDSGAMP YAQWHLLTDE TGEHALPGTE ANLRQFERHF 

       610        620        630        640        650        660 
PADFISEAID QTRGWFYSLH AIATMLYDQP AYRNVICLGH IVDEHGAKMS KSKGNVVEPL 

       670        680        690        700        710        720 
PLFDRYGADS VRWYMFMASD PGDQKRFSER LVAEAQRNYV NTLWNVYAFF VLYANLDRPE 

       730        740        750        760        770        780 
LGAAPAVAER PEMDRWLLAR LEETVRDVTT SLDAYDARGG GRALERFVHD LSNWYVRRNR 

       790        800        810        820        830        840 
SRFWGEGGQV DLSAYATLHE ALLTVSQLTA PFTPFLAEAL YRNLTRGEGA LSVHLTRWPQ 

       850        860        870        880        890        900 
VRPERLDERL TAEMAAVMKV VELGRAVRGA HNLKTRQPLA SVTVRASTPE LTEALRRAQE 

       910        920        930        940        950        960 
QLKEELNVKQ VIFLEGVTDL VRYSLRPNLP VLGKVYGKAL PAVRAALEQA DAAAVAHAVQ 

       970        980        990       1000       1010       1020 
RGERFTVAAN GQTFTLTPEQ VLVDAKAPEG VAAAEDGGFL VAFDTQLTPE LLREGLARDL 

      1030       1040       1050       1060       1070       1080 
VRGIQEARKA AGFEVQDRIR LALDLTGDAR EAAETWRDFI AGEVLATELT FGPGEGYAAA 

      1090 
VEGGTAYLLR H

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References

[1]"Complete sequence of chromosome 1 of Deinococcus geothermalis DSM 11300."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Daly M.J., Fredrickson J.K., Makarova K.S., Gaidamakova E.K., Zhai M., Richardson P.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000359 Genomic DNA. Translation: ABF45399.1.
RefSeqYP_604568.1. NC_008025.1.

3D structure databases

ProteinModelPortalQ1IZD5.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ1IZD5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4058971.
GenomeReviewsGene locus Dgeo_1101 in contig CP000359_GR.
KEGGdge:Dgeo_1101.
NMPDRfig|68909.1.peg.575.
PATRIC21624083. VBIDeiGeo41128_1678.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHBG577712.
OMAPYITYDN.
PhylomeDBQ1IZD5.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ1IZD5_DEIGD
AccessionPrimary (citable) accession number: Q1IZD5
Entry history
Integrated into UniProtKB/TrEMBL: June 13, 2006
Last sequence update: June 13, 2006
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info