Reviewed, UniProtKB/Swiss-Prot Q1IYR1 (NADE_DEIGD)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Dgeo_1327

Organism

Deinococcus geothermalis (strain DSM 11300) [Complete proteome] [HAMAP]

Taxonomic identifier

319795 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077548
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IYR1-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 71589EC63F2AAFF8	FASTA	288	31,320
	10 20 30 40 50 60 MLTLRDRIRQ ELHVRPEIDP GAEVERRVAF LAKYLQHTPA RGFVLGISGG QDSTLAGRLS 70 80 90 100 110 120 QLAAERVRAE GGDATFFAVR LPYGVQADEA DAQTALGFIR PDRTLTVNIK AAVDASARAV 130 140 150 160 170 180 AEALGTGLHN APQPETTPHD PLRDFVRGNI KARERMVAQY AIAGQENLLV VGTDHAAEAL 190 200 210 220 230 240 TGFFTKYGDG GVDLTPLTGL TKRQGAQLLA FLGAPESTWR KVPTADLEDD RPGLPDEVAL 250 260 270 280 GVTYAQIDAY LEGRAVSPEV AARLERLYLA TRHKRALPVT PFDHWWQA

References

[1]

"Complete sequence of chromosome 1 of Deinococcus geothermalis DSM 11300." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Daly M.J., Fredrickson J.K., Makarova K.S., Gaidamakova E.K., Zhai M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000359 Genomic DNA. Translation: ABF45623.1.
RefSeq	YP_604792.1.
3D structure databases
SMR	Q1IYR1. Positions 4-287.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IYR1.
Genome annotation databases
GeneID	4056960.
GenomeReviews	Gene locus Dgeo_1327 in contig CP000359_GR.
KEGG	dge:Dgeo_1327.
NMPDR	fig|68909.1.peg.1254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	HLYEKAP.
PhylomeDB	Q1IYR1.
Enzyme and pathway databases
BioCyc	DGEO319795:DGEO_1327-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_DEIGD

Accession

Primary (citable) accession number: Q1IYR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 13, 2006
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)